An engineered periosteum for efficient delivery of rhBMP-2 and mesenchymal progenitor cells during bone regeneration

During bone regeneration, the periosteum acts as a carrier for key regenerative cues, delivering osteochondroprogenitor cells and crucial growth factors to the injured bone. We developed a biocompatible, 3D polycaprolactone (PCL) melt electro-written membrane to act as a mimetic periosteum. Poly (et...

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Published in:npj Regenerative medicine 2023-09, Vol.8 (1), p.54-54, Article 54
Main Authors: Romero-Torrecilla, Juan Antonio, Lamo-Espinosa, José María, Ripalda-Cemboráin, Purificación, López-Martínez, Tania, Abizanda, Gloria, Riera-Álvarez, Luis, de Galarreta-Moriones, Sergio Ruiz, López-Barberena, Asier, Rodríguez-Flórez, Naiara, Elizalde, Reyes, Jayawarna, Vineetha, Valdés-Fernández, José, de Anleo, Miguel Echanove-González, Childs, Peter, de Juan-Pardo, Elena, Salmeron-Sanchez, Manuel, Prósper, Felipe, Muiños-López, Emma, Granero-Moltó, Froilán
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Language:English
Subjects:
631/61/2035
631/61/490
631/61/54
692/308/575
Biomaterials
Biomedical and Life Sciences
Biomedicine
Bone surgery
Bones
Cell Biology
Collagen
Defects
Fractures
Immunology
Medical research
Orthopedics
Regenerative medicine
Regenerative Medicine/Tissue Engineering
Scanning electron microscopy
Skin & tissue grafts
Stem Cells
Trauma
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cited_by cdi_FETCH-LOGICAL-c518t-439a284bcef3c96362a76eb8ba4edb070a9713ef2e2361762bd4855dcf1cac743
cites cdi_FETCH-LOGICAL-c518t-439a284bcef3c96362a76eb8ba4edb070a9713ef2e2361762bd4855dcf1cac743
container_end_page 54
container_issue 1
container_start_page 54
container_title npj Regenerative medicine
container_volume 8
creator Romero-Torrecilla, Juan Antonio
Lamo-Espinosa, José María
Ripalda-Cemboráin, Purificación
López-Martínez, Tania
Abizanda, Gloria
Riera-Álvarez, Luis
de Galarreta-Moriones, Sergio Ruiz
López-Barberena, Asier
Rodríguez-Flórez, Naiara
Elizalde, Reyes
Jayawarna, Vineetha
Valdés-Fernández, José
de Anleo, Miguel Echanove-González
Childs, Peter
de Juan-Pardo, Elena
Salmeron-Sanchez, Manuel
Prósper, Felipe
Muiños-López, Emma
Granero-Moltó, Froilán
description During bone regeneration, the periosteum acts as a carrier for key regenerative cues, delivering osteochondroprogenitor cells and crucial growth factors to the injured bone. We developed a biocompatible, 3D polycaprolactone (PCL) melt electro-written membrane to act as a mimetic periosteum. Poly (ethyl acrylate) coating of the PCL membrane allowed functionalization, mediated by fibronectin and low dose recombinant human BMP-2 (rhBMP-2) (10-25 μg/ml), resulting in efficient, sustained osteoinduction in vitro. In vivo, rhBMP-2 functionalized mimetic periosteum demonstrated regenerative potential in the treatment of rat critical-size femoral defects with highly efficient healing and functional recovery (80%-93%). Mimetic periosteum has also proven to be efficient for cell delivery, as observed through the migration of transplanted periosteum-derived mesenchymal cells to the bone defect and their survival. Ultimately, mimetic periosteum demonstrated its ability to deliver key stem cells and morphogens to an injured site, exposing a therapeutic and translational potential in vivo when combined with unprecedentedly low rhBMP-2 doses.
doi_str_mv 10.1038/s41536-023-00330-2
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subjects 631/61/2035
631/61/490
631/61/54
692/308/575
Biomaterials
Biomedical and Life Sciences
Biomedicine
Bone surgery
Bones
Cell Biology
Collagen
Defects
Fractures
Immunology
Medical research
Orthopedics
Regenerative medicine
Regenerative Medicine/Tissue Engineering
Scanning electron microscopy
Skin & tissue grafts
Stem Cells
Trauma
title An engineered periosteum for efficient delivery of rhBMP-2 and mesenchymal progenitor cells during bone regeneration
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