Development of Clonal Micropropagation Technology for Ludisia Discolor (Ker Gawl.) A. Rich. In Vitro Conditions

The features of clonal micropropagation in three variations of Ludisia discolor were studied . Brood buds, apical shoot meristems, immature seeds and mature seeds were used as explants. They are grown using Murashige & Skoog modified medium. A special technique has been developed for the sterili...

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Bibliographic Details
Published in:BIO web of conferences 2023-01, Vol.57, p.3003
Main Authors: Nazarov, Vladimir, Semenova, Ekaterina, Andronova, Elena, Tsokalo, Inna, Semenova, Elena
Format: Article
Language:English
Subjects:
Discoloration
Explants
Germination
Leaves
Meristems
Micropropagation
Planting
Seeds
Shoots
Sterilization
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Summary:The features of clonal micropropagation in three variations of Ludisia discolor were studied . Brood buds, apical shoot meristems, immature seeds and mature seeds were used as explants. They are grown using Murashige & Skoog modified medium. A special technique has been developed for the sterilization and planting of microscopic mature seeds. Mature seeds germinated for 60-70 days. Seeds from 25-day-old capsules showed the best results among immature seeds. They gave mass shoots already on the 25-30 day after planting. The roller of the future first leaf of the protocorms was laid in the dark 2 months after germination. Switching to the light mode induced rapid gemmorizogenesis - the formation of the first green leaves, stem and adventitious roots. Seeds from 15-day-old capsules did not germinate at all. Planting with immature seeds is the most effective method of clonal reproduction of L. discolor in vitro .
ISSN:2117-4458
2273-1709
2117-4458
DOI:10.1051/bioconf/20235703003