Rapid Detection of Escherichia coli O157 and Shiga Toxins by Lateral Flow Immunoassays

Shiga toxin-producing Escherichia coli O157:H7 (STEC) cause food-borne illness that may be fatal. STEC strains enumerate two types of potent Shiga toxins (Stx1 and Stx2) that are responsible for causing diseases. It is important to detect the E. coli O157 and Shiga toxins in food to prevent outbreak...

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Bibliographic Details
Published in:Toxins 2016-03, Vol.8 (4), p.92-92
Main Authors: Wang, Jinliang, Katani, Robab, Li, Lingling, Hegde, Narasimha, Roberts, Elisabeth L, Kapur, Vivek, DebRoy, Chitrita
Format: Article
Language:English
Subjects:
Antibodies, Monoclonal - chemistry
Antibodies, Monoclonal - immunology
Bacterial Load
colloidal gold
E coli
E. coli O157
Escherichia coli
Escherichia coli O157 - immunology
Escherichia coli O157 - isolation & purification
Food
food safety
Foodborne diseases
Gold - chemistry
Immunoassay
Immunoassays
lateral flow immunoassay (LFIA)
Metal Nanoparticles - chemistry
Shiga toxin
Shiga Toxin 1 - analysis
Shiga Toxin 1 - immunology
Shiga Toxin 2 - analysis
Shiga Toxin 2 - immunology
STEC
Stx
Toxins
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Summary:Shiga toxin-producing Escherichia coli O157:H7 (STEC) cause food-borne illness that may be fatal. STEC strains enumerate two types of potent Shiga toxins (Stx1 and Stx2) that are responsible for causing diseases. It is important to detect the E. coli O157 and Shiga toxins in food to prevent outbreak of diseases. We describe the development of two multi-analyte antibody-based lateral flow immunoassays (LFIA); one for the detection of Stx1 and Stx2 and one for the detection of E. coli O157 that may be used simultaneously to detect pathogenic E. coli O157:H7. The LFIA strips were developed by conjugating nano colloidal gold particles with monoclonal antibodies against Stx1 and Stx2 and anti-lipid A antibodies to capture Shiga toxins and O157 antigen, respectively. Our results indicate that the LFIA for Stx is highly specific and detected Stx1 and Stx2 within three hours of induction of STEC with ciprofloxacin at 37 °C. The limit of detection for E. coli O157 LFIA was found to be 10⁵ CFU/mL in ground beef spiked with the pathogen. The LFIAs are rapid, accurate and easy to use and do not require sophisticated equipment or trained personnel. Following the assay, colored bands on the membrane develop for end-point detection. The LFIAs may be used for screening STEC in food and the environment.
ISSN:2072-6651
2072-6651
DOI:10.3390/toxins8040092