Tolerance enhancement of Dendrobium officinale by salicylic acid family-related metabolic pathways under unfavorable temperature

• Published in: BMC plant biology 2024-08, Vol.24 (1), p.770-770, Article 770
• Main Authors: Wang, Wenhua, Zheng, Mingqiong, Shen, Zhijun, Meng, Hongyan, Chen, Lianghua, Li, Tiantian, Lin, Fucong, Hong, Liping, Lin, Zhikai, Ye, Ting, Guo, Ying, He, Enming
• Format: Article
• Language: English
• Subjects: Acid resistance; Amino acids; Analogs; Benzamide; Benzoic acid; Bioflavonoids; Botanical research; Chlorophyll; Chlorophyll - metabolism; Chlorophyll fluorescence imaging; Cold; Crystal structure; Dendrobium - drug effects; Dendrobium - metabolism; Dendrobium officinale; energy; Extreme values; family; Flavones; Flavonoids; Fluorescence; Food quality; Force and energy; guanosine monophosphate; Hardiness; Heat; Hydroxycinnamic acid; Impact analysis; Leaves; Metabolic Networks and Pathways - drug effects; Metabolic pathways; Metabolism; Metabolites; metabolomics; Molecular docking; Molecular Docking Simulation; Molecular structure; NPR4 protein; Orchids; Parameter identification; Parameter sensitivity; Pharmacology; Phenols; Physiological aspects; Physiology; Plant heat tolerance; Plant Proteins - metabolism; Plants; Position indicators; Production processes; Protein binding; Protein structure; Proteins; Salicylic acid; Salicylic Acid - metabolism; Salicylic Acid - pharmacology; Salicylic acid family; Signal transduction; Temperature; Temperature tolerance; therapeutics
• ISSN: 1471-2229; 1471-2229
• DOI: 10.1186/s12870-024-05499-2

Unfavorable temperatures significantly constrain the quality formation of Dendrobium officinale, severely limiting its food demand. Salicylic acid (SA) enhances the resistance of D. officinale to stress and possesses various analogs. The impact and mechanism of the SA family on improving the quality of D. officinale under adverse temperature conditions remains unclear. Combined with molecular docking analysis, chlorophyll fluorescence and metabolic analysis after treatments with SA analogues or extreme temperatures are performed in this study. The results demonstrate that both heat and cold treatments impede several main parameters of chlorophyll fluorescence of D. officinale, including the ΦPSII parameter, a sensitive growth indicator. However, this inhibition is mitigated by SA or its chemically similar compounds. Comprehensive branch imaging of ΦPSII values revealed position-dependent improvement of tolerance. Molecular docking analysis using a crystal structure model of NPR4 protein reveals that the therapeutic effects of SA analogs are determined by their binding energy and the contact of certain residues. Metabolome analysis identifies 17 compounds are considered participating in the temperature-related SA signaling pathway. Moreover, several natural SA analogs such as 2-hydroxycinnamic acid, benzamide, 2-(formylamino) benzoic acid and 3-o-methylgallic acid, are further found to have high binding ability to NPR4 protein and probably enhance the tolerance of D. officinale against unfavorable temperatures through flavone and guanosine monophosphate degradation pathways. These results reveal that the SA family with a high binding capability of NPR4 could improve the tolerance of D. officinale upon extreme temperature challenges. This study also highlights the collaborative role of SA-related natural compounds present in D. officinale in the mechanism of temperature resistance and offers a potential way to develop protective agents for the cultivation of D. officinale.