Ganoderma Fusions with High Yield of Ergothioneine and Comparative Analysis of Its Genomics

Ergothioneine (EGT), an exceptional antioxidant found ubiquitously across diverse living organisms, plays a pivotal role in various vital physiological regulatory functions. Its principal natural sources are mushrooms and animal liver tissues. Ganoderma spp., a traditional Chinese food and medicinal...

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Bibliographic Details
Published in:Journal of fungi (Basel) 2023-11, Vol.9 (11), p.1072
Main Authors: Xie, Jiaqi, Yu, Yinghao, You, Junjiang, Ye, Zhiwei, Zhou, Fenglong, Wang, Na, Zhong, Jingru, Guo, Liqiong, Lin, Junfang
Format: Article
Language:English
Subjects:
Comparative analysis
Enzymes
ergothioneine
Fruit bodies
Ganoderma
Ganoderma fusions
genome analysis
Genomes
Genomic analysis
Mushrooms
Mutation
Mycelia
protoplast fusion
Strains (organisms)
Sucrose
Traditional Chinese medicine
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Summary:Ergothioneine (EGT), an exceptional antioxidant found ubiquitously across diverse living organisms, plays a pivotal role in various vital physiological regulatory functions. Its principal natural sources are mushrooms and animal liver tissues. Ganoderma spp., a traditional Chinese food and medicinal mushroom, boasts high concentrations of EGT. To advance the development of novel Ganoderma spp. strains with enhanced EGT yields, we employed an efficient Ganoderma spp. protoplasmic fusion system. Through molecular and biological characterization, we successfully generated seven novel fusion strains. Notably, fusion strain RS7 demonstrated a remarkable increase in mycelial EGT production (12.70 ± 1.85 mg/L), surpassing the parental strains FQ16 and FQ23 by 34.23% and 39.10%, respectively. Furthermore, in the context of the fruiting body, fusion strain RS11 displayed a notable 53.58% enhancement in EGT production (11.24 ± 1.96 mg/L) compared to its parental strains. Genomic analysis of the RS7, the strain with the highest levels of mycelial EGT production, revealed mutations in the gene EVM0005141 associated with EGT metabolism. These mutations led to a reduction in non-productive shunts, subsequently redirecting more substrate towards the EGT synthesis pathway. This redirection significantly boosted EGT production in the RS7 strain. The insights gained from this study provide valuable guidance for the commercial-scale production of EGT and the selective breeding of Ganoderma spp. strains.
ISSN:2309-608X
2309-608X
DOI:10.3390/jof9111072