The Development of New Primer Sets for the Amplification and Sequencing of the Envelope Gene of All Dengue Virus Serotypes

Dengue virus (DENV) poses a significant threat to global health, infecting approximately 390 million people annually. This virus comprises four serotypes capable of causing severe disease. Genetic analyses are crucial for understanding the epidemiology, evolution, and spread of DENV. Although previo...

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Bibliographic Details
Published in:Microorganisms (Basel) 2024-05, Vol.12 (6), p.1092
Main Authors: Fraenkel, Stefania, Nabeshima, Takeshi, Xayavong, Dalouny, Nguyen, Thi Thanh Ngan, Xu, Qiang, Kapandji, Merveille, Yamao, Kano, Balingit, Jean Claude, Pandey, Basu Dev, Morita, Kouichi, Hasebe, Futoshi, Ngwe Tun, Mya Myat, Takamatsu, Yuki
Format: Article
Language:English
Subjects:
Amplification
Dengue fever
Dengue viruses
DENV
Design
Env gene
Env protein
Epidemics
Epidemiology
Evolutionary genetics
Genes
Genetic analysis
Genetic diversity
Genomes
Global health
Health aspects
Mosquitoes
Primers
Proteins
Public health
RT-PCR
sequencing
serotype-specific
Serotypes
Tropical diseases
Vector-borne diseases
Viral envelope proteins
Viruses
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Summary:Dengue virus (DENV) poses a significant threat to global health, infecting approximately 390 million people annually. This virus comprises four serotypes capable of causing severe disease. Genetic analyses are crucial for understanding the epidemiology, evolution, and spread of DENV. Although previous studies have focused on the envelope protein-coding (E) gene, only a few primers can efficiently detect and amplify the viral genes from multiple endemic countries simultaneously. In this study, we designed degenerate primer pairs for each DENV serotype to amplify and sequence the entire E gene, using globally representative sequences for each serotype. These primers were validated using DENV isolates from various Asian countries and demonstrated broad-spectrum detection capabilities and high-quality sequences. The primers provide effective tools for genetic analysis in the regions affected by dengue, aiding strain identification and epidemiological studies during outbreaks.
ISSN:2076-2607
2076-2607
DOI:10.3390/microorganisms12061092