A Comparative Study of Newly Developed HPLC-DAD and UHPLC-UV Assays for the Determination of Posaconazole in Bulk Powder and Suspension Dosage Form

• Published in: Journal of analytical methods in chemistry 2014-01, Vol.2014 (2014), p.1-7
• Main Authors: Hamdy, Dalia A., Belal, Tarek S.
• Format: Article
• Language: English
• Subjects: Acetonitrile; Analysis; Assaying; Biochemical assays; Calibration; Chemical properties; Chemotherapy; Chromatography; Dosage; Drug dosages; Economics; Guidelines; High performance liquid chromatography; Mass spectrometry; Mathematical analysis; Methods; Partially stabilized zirconia; Pharmaceutical industry; Posaconazole; Potassium; Quality control; Sensors
• ISSN: 2090-8865; 2090-8873
• DOI: 10.1155/2014/241035

Objective. To develop and compare HPLC-DAD and UHPLC-UV assays for the quantitation of posaconazole in bulk powder and suspension dosage form. Methods. Posaconazole linearity range was 5–50 μg/mL for both assays. For HPLC-DAD assay, samples were injected through Zorbax SB-C18 (4.6 × 250 mm, 5 μm) column. The gradient elution composed of the mobile phase acetonitrile: 15 mM potassium dihydrogen orthophosphate (30 : 70 to 80 : 20, linear over 7 minutes) pumped at 1.5 mL/min. For UHPLC-UV assay, samples were injected through Kinetex-C18 (2.1 × 50 mm, 1.3 μm) column. The mobile phase composed of acetonitrile: 15 mM potassium dihydrogen orthophosphate (45 : 55) pumped isocratically at 0.4 mL/min. Detection wavelength was 262 nm in both methods. Results. The run time was 11 and 3 minutes for HPLC-DAD and UHPLC-UV assays, respectively. Both assays were linear ( r 2 > 0.999 ) with CV% and % error of the mean