Endophytic Bacterial Biofilm-Formers Associated with Antarctic Vascular Plants

• Published in: Microorganisms (Basel) 2024-09, Vol.12 (10), p.1938
• Main Authors: Iungin, Olga, Prekrasna-Kviatkovska, Yevheniia, Kalinichenko, Oleksandr, Moshynets, Olena, Potters, Geert, Sidorenko, Marina, Savchuk, Yaroslav, Mickevičius, Saulius
• Format: Article
• Language: English
• Subjects: Abiotic stress; Adaptation; Ammonia; amyloids; Bacteria; bacterial endophytes; biofilm; Biofilms; Carbon; Deschampsia antarctica Desv; Developmental plasticity; Ecological adaptation; Endophytes; Environmental aspects; Extreme environments; Flowers & plants; Fungicides; Hafnium oxide; Host plants; Leaves; Microbial mats; Microbiota; Microorganisms; Nitrogen fixation; Nitrogenation; Oligotrophy; PGPB; Physiological aspects; Physiology; Plant growth; Plant layout; Plant tissues; plant–microbial interactions; R&D; Research & development; rRNA 16S; Salinity tolerance; Solubilization; Strains (organisms); Temperature tolerance; Vascular plants
• ISSN: 2076-2607; 2076-2607
• DOI: 10.3390/microorganisms12101938

and are the only two vascular plants colonized on the Antarctic continent, which is usually exposed to extreme environments. Endophytic bacteria residing within plant tissues can exhibit diverse adaptations that contribute to their ecological success and potential benefits for their plant hosts. This study aimed to characterize 12 endophytic bacterial strains isolated from these plants, focusing on their ecological adaptations and functional roles like plant growth promotion, antifungal activities, tolerance to salt and low-carbon environments, wide temperature range, and biofilm formation. Using 16S rRNA sequencing, we identified several strains, including novel species like Hafnia and Agreia. Many strains exhibited nitrogen-fixing ability, phosphate solubilization, ammonia, and IAA production, potentially benefiting their hosts. Additionally, halotolerance and carbon oligotrophy were also shown by studied bacteria. While some Antarctic bacteria remain strictly psychrophilic, others demonstrate a remarkable ability to tolerate a wider range of temperatures, suggesting that they have acquired mechanisms to cope with fluctuations in environmental temperature and developed adaptations to survive in intermediate hosts like mammals and/or birds. Such adaptations and high plasticity of metabolism of Antarctic endophytic bacteria provide a foundation for research and development of new promising products or mechanisms for use in agriculture and technology.