19F MRI-fluorescence imaging dual-modal cell tracking with partially fluorinated nanoemulsions

As a noninvasive “hot-spot” imaging technology, fluorine-19 magnetic resonance imaging ( 19 F MRI) has been extensively used in cell tracking. However, the peculiar physicochemical properties of perfluorocarbons (PFCs), the most commonly used 19 F MRI agents, sometimes cause low sensitivity, poor ce...

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Bibliographic Details
Published in:Frontiers in bioengineering and biotechnology 2022-11, Vol.10, p.1049750-1049750
Main Authors: Tang, Ting, Zhu, Qiang, Liu, Shuang, Dai, Hailong, Li, Yu, Tang, Caihong, Chen, Kexin, Jiang, Mou, Zhu, Lijun, Zhou, Xin, Chen, ShiZhen, Zheng, Zitong, Jiang, Zhong-Xing
Format: Article
Language:English
Subjects:
19F MRI
Bioengineering and Biotechnology
cell tracking
fluorescence
nanoemulsions
partially fluorinated
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Summary:As a noninvasive “hot-spot” imaging technology, fluorine-19 magnetic resonance imaging ( 19 F MRI) has been extensively used in cell tracking. However, the peculiar physicochemical properties of perfluorocarbons (PFCs), the most commonly used 19 F MRI agents, sometimes cause low sensitivity, poor cell uptake, and misleading results. In this study, a partially fluorinated agent, perfluoro- tert -butyl benzyl ether, was used to formulate a 19 F MRI-fluorescence imaging (FLI) dual-modal nanoemulsion for cell tracking. Compared with PFCs, the partially fluorinated agent showed considerably improved physicochemical properties, such as lower density, shorter longitudinal relaxation times, and higher solubility to fluorophores, while maintaining high 19 F MRI sensitivity. After being formulated into stable, monodisperse, and paramagnetic Fe 3+ -promoted nanoemulsions, the partially fluorinated agent was used in 19 F MRI-FLI dual imaging tracking of lung cancer A549 cells and macrophages in an inflammation mouse model.
ISSN:2296-4185
2296-4185
DOI:10.3389/fbioe.2022.1049750