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Type VI secretion system killing by commensal Neisseria is influenced by expression of type four pili
Type VI Secretion Systems (T6SSs) are widespread in bacteria and can dictate the development and organisation of polymicrobial ecosystems by mediating contact dependent killing. In species, including a commensal of the human respiratory tract, interbacterial contacts are mediated by Type four pili (...
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| Published in: | eLife 2021-07, Vol.10 |
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| Main Authors: | , , , , , , |
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| creator | Custodio, Rafael Ford, Rhian M Ellison, Cara J Liu, Guangyu Mickute, Gerda Tang, Christoph M Exley, Rachel M |
| description | Type VI Secretion Systems (T6SSs) are widespread in bacteria and can dictate the development and organisation of polymicrobial ecosystems by mediating contact dependent killing. In
species, including
a commensal of the human respiratory tract, interbacterial contacts are mediated by Type four pili (Tfp) which promote formation of aggregates and govern the spatial dynamics of growing
microcolonies. Here, we show that
expresses a plasmid-encoded T6SS that is active and can limit growth of related pathogens. We explored the impact of Tfp on
T6SS-dependent killing within a colony and show that pilus expression by a prey strain enhances susceptibility to T6SS compared to a non-piliated prey, by preventing segregation from a T6SS-wielding attacker. Our findings have important implications for understanding how spatial constraints during contact-dependent antagonism can shape the evolution of microbial communities. |
| doi_str_mv | 10.7554/eLife.63755 |
| format | article |
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species, including
a commensal of the human respiratory tract, interbacterial contacts are mediated by Type four pili (Tfp) which promote formation of aggregates and govern the spatial dynamics of growing
microcolonies. Here, we show that
expresses a plasmid-encoded T6SS that is active and can limit growth of related pathogens. We explored the impact of Tfp on
T6SS-dependent killing within a colony and show that pilus expression by a prey strain enhances susceptibility to T6SS compared to a non-piliated prey, by preventing segregation from a T6SS-wielding attacker. Our findings have important implications for understanding how spatial constraints during contact-dependent antagonism can shape the evolution of microbial communities.</description><identifier>ISSN: 2050-084X</identifier><identifier>EISSN: 2050-084X</identifier><identifier>DOI: 10.7554/eLife.63755</identifier><identifier>PMID: 34232858</identifier><language>eng</language><publisher>England: eLife Science Publications, Ltd</publisher><subject>Antagonism ; Bacteria ; Competition ; Ecosystems ; Fimbriae, Bacterial - metabolism ; Genes ; Genomes ; Microbiology and Infectious Disease ; Microbiota - physiology ; Neisseria ; Neisseria cinerea ; Neisseria cinerea - physiology ; Neisseria meningitidis ; Pathogens ; Pili ; Prey ; Respiratory tract ; Sea lions ; Secretion ; Symbiosis - genetics ; T6SS ; Type IV pili ; Type VI Secretion Systems - metabolism</subject><ispartof>eLife, 2021-07, Vol.10</ispartof><rights>2021, Custodio et al.</rights><rights>COPYRIGHT 2021 eLife Science Publications, Ltd.</rights><rights>2021, Custodio et al. This work is published under https://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2021, Custodio et al 2021 Custodio et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c576t-79fbf3b02922c0797e5ad8eb3a5bb8c9e624eda3e0607b1249c4a78171b283ed3</citedby><cites>FETCH-LOGICAL-c576t-79fbf3b02922c0797e5ad8eb3a5bb8c9e624eda3e0607b1249c4a78171b283ed3</cites><orcidid>0000-0002-2477-7565 ; 0000-0001-8366-3245 ; 0000-0002-7561-5515 ; 0000-0001-9120-5586</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/2595207854/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2595207854?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,25753,27924,27925,37012,37013,44590,53791,53793,75126</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34232858$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Custodio, Rafael</creatorcontrib><creatorcontrib>Ford, Rhian M</creatorcontrib><creatorcontrib>Ellison, Cara J</creatorcontrib><creatorcontrib>Liu, Guangyu</creatorcontrib><creatorcontrib>Mickute, Gerda</creatorcontrib><creatorcontrib>Tang, Christoph M</creatorcontrib><creatorcontrib>Exley, Rachel M</creatorcontrib><title>Type VI secretion system killing by commensal Neisseria is influenced by expression of type four pili</title><title>eLife</title><addtitle>Elife</addtitle><description>Type VI Secretion Systems (T6SSs) are widespread in bacteria and can dictate the development and organisation of polymicrobial ecosystems by mediating contact dependent killing. In
species, including
a commensal of the human respiratory tract, interbacterial contacts are mediated by Type four pili (Tfp) which promote formation of aggregates and govern the spatial dynamics of growing
microcolonies. Here, we show that
expresses a plasmid-encoded T6SS that is active and can limit growth of related pathogens. We explored the impact of Tfp on
T6SS-dependent killing within a colony and show that pilus expression by a prey strain enhances susceptibility to T6SS compared to a non-piliated prey, by preventing segregation from a T6SS-wielding attacker. 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In
species, including
a commensal of the human respiratory tract, interbacterial contacts are mediated by Type four pili (Tfp) which promote formation of aggregates and govern the spatial dynamics of growing
microcolonies. Here, we show that
expresses a plasmid-encoded T6SS that is active and can limit growth of related pathogens. We explored the impact of Tfp on
T6SS-dependent killing within a colony and show that pilus expression by a prey strain enhances susceptibility to T6SS compared to a non-piliated prey, by preventing segregation from a T6SS-wielding attacker. Our findings have important implications for understanding how spatial constraints during contact-dependent antagonism can shape the evolution of microbial communities.</abstract><cop>England</cop><pub>eLife Science Publications, Ltd</pub><pmid>34232858</pmid><doi>10.7554/eLife.63755</doi><orcidid>https://orcid.org/0000-0002-2477-7565</orcidid><orcidid>https://orcid.org/0000-0001-8366-3245</orcidid><orcidid>https://orcid.org/0000-0002-7561-5515</orcidid><orcidid>https://orcid.org/0000-0001-9120-5586</orcidid><oa>free_for_read</oa></addata></record> |
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| source | Open Access: PubMed Central; Publicly Available Content Database |
| subjects | Antagonism Bacteria Competition Ecosystems Fimbriae, Bacterial - metabolism Genes Genomes Microbiology and Infectious Disease Microbiota - physiology Neisseria Neisseria cinerea Neisseria cinerea - physiology Neisseria meningitidis Pathogens Pili Prey Respiratory tract Sea lions Secretion Symbiosis - genetics T6SS Type IV pili Type VI Secretion Systems - metabolism |
| title | Type VI secretion system killing by commensal Neisseria is influenced by expression of type four pili |
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