Iron-Imprinted Single-Atomic Site Catalyst-Based Nanoprobe for Detection of Hydrogen Peroxide in Living Cells

• Published in: Nano-micro letters 2021-12, Vol.13 (1), p.146-146, Article 146
• Main Authors: Lyu, Zhaoyuan, Ding, Shichao, Wang, Maoyu, Pan, Xiaoqing, Feng, Zhenxing, Tian, Hangyu, Zhu, Chengzhou, Du, Dan, Lin, Yuehe
• Format: Article
• Language: English
• Subjects: Alzheimer's disease; Biosensing; Biosensors; Breast cancer; Catalysts; Chemical synthesis; Colorimetry; Engineering; Enzymes; Hydrogen peroxide; Iron; Living cell; Nanoprobe; Nanoscale Science and Technology; Nanotechnology; Nanotechnology and Microengineering; Peroxidase; Peroxidase-like activities; Physiology; Polymerization; Single-atomic site catalysts
• ISSN: 2311-6706; 2150-5551
• DOI: 10.1007/s40820-021-00661-z

Highlights A facile ion-imprinting method (IIM) is used to synthesize the isolated Fe-N-C single-atomic site catalyst (IIM-Fe-SASC), which mimics the natural enzyme-like active site and shows excellent peroxidase-like activity. The ion-imprinting process can precisely control ion at the atomic level and form numerous well-defined single-atomic Fe-N-C sites. The IIM-Fe-SASC has been successfully used as the nanoprobe for in situ H 2 O 2 detection generated from MDA-MB-231 cells. Fe-based single-atomic site catalysts (SASCs), with the natural metalloproteases-like active site structure, have attracted widespread attention in biocatalysis and biosensing. Precisely, controlling the isolated single-atom Fe-N-C active site structure is crucial to improve the SASCs’ performance. In this work, we use a facile ion-imprinting method (IIM) to synthesize isolated Fe-N-C single-atomic site catalysts (IIM-Fe-SASC). With this method, the ion-imprinting process can precisely control ion at the atomic level and form numerous well-defined single-atomic Fe-N-C sites. The IIM-Fe-SASC shows better peroxidase-like activities than that of non-imprinted references. Due to its excellent properties, IIM-Fe-SASC is an ideal nanoprobe used in the colorimetric biosensing of hydrogen peroxide (H 2 O 2 ). Using IIM-Fe-SASC as the nanoprobe, in situ detection of H 2 O 2 generated from MDA-MB-231 cells has been successfully demonstrated with satisfactory sensitivity and specificity. This work opens a novel and easy route in designing advanced SASC and provides a sensitive tool for intracellular H 2 O 2 detection.