Modulation of the Activities of Catalase, Cu-Zn, Mn Superoxide Dismutase, and Glutathione Peroxidase in Adipocyte from Ovariectomised Female Rats with Metabolic Syndrome

The aim of this study was to evaluate the association between estrogen removal, antioxidant enzymes, and oxidative stress generated by obesity in a MS female rat model. Thirty two female Wistar rats were divided into 4 groups: Control (C), MS, MS ovariectomized (Ovx), and MS Ovx plus estradiol (E2)....

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Bibliographic Details
Published in:International Journal of Endocrinology 2014-01, Vol.2014 (2014), p.168-177-018
Main Authors: Guerra, Rebeca Cambray, Zuñiga-Muñoz, Alejandra, Guarner Lans, Verónica, Díaz-Díaz, Eulises, Tena Betancourt, Carlos Alberto, Pérez-Torres, Israel
Format: Article
Language:English
Subjects:
Abdomen
Adipocytes
Adipose tissues
Antioxidants
Complications and side effects
Cytokines
Endocrinology
Enzymes
Estrogens
Females
Genetic aspects
Hormones
Hypertension
Insulin resistance
Laboratory animals
Metabolic syndrome
Metabolic syndrome X
Obesity
Oxidative stress
Peroxidase
Physiological aspects
Rodents
Sucrose
Superoxide dismutase
Triglycerides
Womens health
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Summary:The aim of this study was to evaluate the association between estrogen removal, antioxidant enzymes, and oxidative stress generated by obesity in a MS female rat model. Thirty two female Wistar rats were divided into 4 groups: Control (C), MS, MS ovariectomized (Ovx), and MS Ovx plus estradiol (E2). MS was induced by administering 30% sucrose to drinking water for 24 weeks. After sacrifice, intra-abdominal fat was dissected; adipocytes were isolated and lipid peroxidation, non-enzymatic antioxidant capacity, and the activities of Cu-Zn and Mn superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) were determined. There were no significant differences in the activities of Cu-Zn, Mn SOD, CAT, and GPx between the C and MS groups, but in the MS Ovx group there was a statistically significant decrease in the activities of these enzymes when compared to MS and MS Ovx+E2. The increased lipid peroxidation and nonenzymatic antioxidant capacity found in MS Ovx was significantly decreased when compared to MS and MS Ovx+E2. In conclusion, the removal of E2 by ovariectomy decreases the activity of the antioxidant enzymes in the intra-abdominal tissue of MS female rats; this is reflected by increased lipid peroxidation and decreased nonenzymatic antioxidant capacity.
ISSN:1687-8337
1687-8345
DOI:10.1155/2014/175080