Downregulation of miR-210 Promoted Apoptosis of Hippocampal Neurons by Negatively Regulating the TLR4/NF-[kappa]B1 Signaling Pathway in a Rat Model of Status Epilepticus

Purpose: Status epilepticus (SE) is a life-threatening condition causing brain damage, hippocampal necrosis and apoptosis. This study aimed to determine whether microRNA-210 regulates seizure and apoptosis by targeting the TLR4 /NF-[kappa]B1 associated signaling pathway. Methods: In a pilocarpine-in...

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Published in:Neuropsychiatric disease and treatment 2022-08, Vol.18, p.1763-1770
Main Authors: Zhou, Qin, Luo, Huanjun, Wang, Xiaowei, Li, Peng, Kong, Haibo, He, Baomei
Format: Article
Language:English
Subjects:
Apoptosis
Cellular signal transduction
Development and progression
Genetic aspects
Health aspects
MicroRNA
Neurological research
Neurons
nf-κb1
Seizures (Medicine)
Status epilepticus
TLR4
Transcription factors
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Summary:Purpose: Status epilepticus (SE) is a life-threatening condition causing brain damage, hippocampal necrosis and apoptosis. This study aimed to determine whether microRNA-210 regulates seizure and apoptosis by targeting the TLR4 /NF-[kappa]B1 associated signaling pathway. Methods: In a pilocarpine-induced epileptic rat model, the expressions of microRNA-210 (miR-210), TLR4, NF-[kappa]B1 and caspase-3 were assessed by a quantitative polymerase chain reaction and Western blotting. Tunel detects hippocampal neuron apoptosis. Results: We found that miR-210, TLR4, NF-[kappa]B1 and caspase-3 were upregulated in the hippocampus of the rat model compared with that of control. The knockdown of miR-210 significantly restored the expression levels of TLR4, NF-[kappa]B1 and caspase-3 and increased hippocampal apoptosis. Conclusion: These findings showed that the downregulation of miR-210 promoted apoptosis of hippocampal neurons by negatively regulating the TLR4/NF-[kappa]B1 signaling pathway. Keywords: apoptosis, microRNA, NF-[kappa]B1, status epilepticus, TLR4
ISSN:1176-6328
1178-2021
DOI:10.2147/NDT.S371950