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Continuous feeding strategy for polyhydroxyalkanoate production from solid waste animal fat at laboratory‐ and pilot‐scale
Bioconversion of waste animal fat (WAF) to polyhydroxyalkanoates (PHAs) is an approach to lower the production costs of these plastic alternatives. However, the solid nature of WAF requires a tailor‐made process development. In this study, a double‐jacket feeding system was built to thermally liquef...
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| Published in: | Microbial biotechnology 2023-02, Vol.16 (2), p.295-306 |
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| creator | Gutschmann, Björn Maldonado Simões, Matilde Schiewe, Thomas Schröter, Edith S. Münzberg, Marvin Neubauer, Peter Bockisch, Anika Riedel, Sebastian L. |
| description | Bioconversion of waste animal fat (WAF) to polyhydroxyalkanoates (PHAs) is an approach to lower the production costs of these plastic alternatives. However, the solid nature of WAF requires a tailor‐made process development. In this study, a double‐jacket feeding system was built to thermally liquefy the WAF to employ a continuous feeding strategy. During laboratory‐scale cultivations with Ralstonia eutropha Re2058/pCB113, 70% more PHA (45 gPHA L−1) and a 75% higher space–time yield (0.63 gPHA L−1 h−1) were achieved compared to previously reported fermentations with solid WAF. During the development process, growth and PHA formation were monitored in real‐time by in‐line photon density wave spectroscopy. The process robustness was further evaluated during scale‐down fermentations employing an oscillating aeration, which did not alter the PHA yield although cells encountered periods of oxygen limitation. Flow cytometry with propidium iodide staining showed that more than two‐thirds of the cells were viable at the end of the cultivation and viability was even little higher in the scale‐down cultivations. Application of this feeding system at 150‐L pilot‐scale cultivation yielded in 31.5 gPHA L−1, which is a promising result for the further scale‐up to industrial scale. |
| doi_str_mv | 10.1111/1751-7915.14104 |
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However, the solid nature of WAF requires a tailor‐made process development. In this study, a double‐jacket feeding system was built to thermally liquefy the WAF to employ a continuous feeding strategy. During laboratory‐scale cultivations with Ralstonia eutropha Re2058/pCB113, 70% more PHA (45 gPHA L−1) and a 75% higher space–time yield (0.63 gPHA L−1 h−1) were achieved compared to previously reported fermentations with solid WAF. During the development process, growth and PHA formation were monitored in real‐time by in‐line photon density wave spectroscopy. The process robustness was further evaluated during scale‐down fermentations employing an oscillating aeration, which did not alter the PHA yield although cells encountered periods of oxygen limitation. Flow cytometry with propidium iodide staining showed that more than two‐thirds of the cells were viable at the end of the cultivation and viability was even little higher in the scale‐down cultivations. Application of this feeding system at 150‐L pilot‐scale cultivation yielded in 31.5 gPHA L−1, which is a promising result for the further scale‐up to industrial scale.</description><identifier>ISSN: 1751-7915</identifier><identifier>EISSN: 1751-7915</identifier><identifier>DOI: 10.1111/1751-7915.14104</identifier><identifier>PMID: 35921398</identifier><language>eng</language><publisher>United States: John Wiley & Sons, Inc</publisher><subject>Aeration ; Animal fat ; Animals ; Batch processes ; Bioconversion ; Bioreactors ; Carbon ; Cultivation ; Feeding ; Fermentation ; Flow cytometry ; Iodides ; Laboratories ; Nitrogen ; Photon density ; Plastics ; Polyhydroxyalkanoates ; Polyhydroxyalkanoates - metabolism ; Polyhydroxyalkanoic acid ; Production costs ; Propidium iodide ; Soil bacteria ; Solid Waste ; Solid wastes ; Spectroscopy ; Spectrum analysis</subject><ispartof>Microbial biotechnology, 2023-02, Vol.16 (2), p.295-306</ispartof><rights>2022 The Authors. published by Society for Applied Microbiology and John Wiley & Sons Ltd.</rights><rights>2022 The Authors. Microbial Biotechnology published by Society for Applied Microbiology and John Wiley & Sons Ltd.</rights><rights>2023. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). 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| ispartof | Microbial biotechnology, 2023-02, Vol.16 (2), p.295-306 |
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| source | Wiley Online Library Open Access; Publicly Available Content Database; PubMed Central |
| subjects | Aeration Animal fat Animals Batch processes Bioconversion Bioreactors Carbon Cultivation Feeding Fermentation Flow cytometry Iodides Laboratories Nitrogen Photon density Plastics Polyhydroxyalkanoates Polyhydroxyalkanoates - metabolism Polyhydroxyalkanoic acid Production costs Propidium iodide Soil bacteria Solid Waste Solid wastes Spectroscopy Spectrum analysis |
| title | Continuous feeding strategy for polyhydroxyalkanoate production from solid waste animal fat at laboratory‐ and pilot‐scale |
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