Comparison between the Counter Immunoelectrophoresis Test and Mouse Neutralization Test for the Detection of Antibodies against Rabies Virus in Dog Sera

The detection of rabies antibodies is extremely valuable for epidemiological studies, determination of immune status in man, animals, and for the diagnosis of the disease. Several serological procedures have been described for this purpose. The present study reports a comparison between counterimmun...

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Bibliographic Details
Published in:Memórias do Instituto Oswaldo Cruz 2002-03, Vol.97 (2), p.259-261
Main Authors: Silva, Luzia Helena Queiroz da, Bissoto, César Eduardo, Carvalho, Cristiano de, Cardoso, Tereza Cristina, Pinheiro, Devani M, Perri, Silvia Helena Venturoli
Format: Article
Language:English
Subjects:
Animals
Antibodies, Viral - blood
Counterimmunoelectrophoresis
counterimmunoelectrophoresis test
Dog Diseases - diagnosis
Dog Diseases - immunology
Dog Diseases - prevention & control
Dogs
Linear Models
Mice
mouse neutralization test
Neutralization Tests
PARASITOLOGY
rabies
Rabies - diagnosis
Rabies - immunology
Rabies - prevention & control
rabies - rabies antibodies - mouse neutralization test - counterimmunoelectrophoresis test
rabies antibodies
Rabies Vaccines - immunology
Rabies virus
Rabies virus - immunology
Sensitivity and Specificity
TROPICAL MEDICINE
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Summary:The detection of rabies antibodies is extremely valuable for epidemiological studies, determination of immune status in man, animals, and for the diagnosis of the disease. Several serological procedures have been described for this purpose. The present study reports a comparison between counterimmunoelectrophoresis test (CIET) and mouse neutralization test (MNT) in the detection of antibodies against rabies virus from 212 serum samples of vaccinated dogs. The agreement between both techniques was 79.7% and a significative association was demonstrated. The correlation coefficients between MNT and the CIET titers was determined considering 88 samples showing positive results in both techniques [CIET = 2 and MNT = 5 (0.13 IU/ml)] and resulted r2 = 0.7926 (p < 0.001). The performance of CIET system was technically simple, cheap and rapid, and thereby it could be useful for serological monitoring of dog vaccination campaigns as well as for individual analysis.
ISSN:1678-8060
0074-0276
0074-0276
1678-8060
DOI:10.1590/s0074-02762002000200020