Diagnostic Accuracy of Leucine-Rich α-2-Glycoprotein 1 as a Non-Invasive Salivary Biomarker in Pediatric Appendicitis

• Published in: International journal of molecular sciences 2023-03, Vol.24 (7), p.6043
• Main Authors: Tintor, Goran, Jukić, Miro, Šupe-Domić, Daniela, Jerončić, Ana, Pogorelić, Zenon
• Format: Article
• Language: English
• Subjects: Abdomen; acute appendicitis; Acute Disease; Amino acids; Appendectomy; Appendicitis; Appendicitis - diagnosis; Appendicitis - surgery; biomarker; Biomarkers; Blood cell count; C-reactive protein; C-Reactive Protein - metabolism; Child; children; Diagnosis; Diagnostic systems; Emergency medical care; Emergency medical services; Enzyme-linked immunosorbent assay; Gangrene; Glycoproteins; Humans; Inflammation; Laboratories; Laparoscopy; Leucine; leucine-rich α-2-glycoprotein 1; Leukocyte Count; Leukocytes (neutrophilic); LRG1; Mass spectrometry; Neutrophils; Patients; Pediatrics; Population; Prospective Studies; Saliva; Scientific imaging; Sensitivity and Specificity; Tumor necrosis factor-TNF; Urine
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms24076043

The aim of this study is to evaluate the diagnostic accuracy of leucine-rich α-2-glycoprotein 1 (LRG1) in saliva as a novel biomarker for acute appendicitis in the pediatric population. From October 2021 to June 2022, 92 children aged 5 to 17 years who presented with acute abdomen and suspected acute appendicitis were enrolled in this prospective study. The parameters documented included demographic and clinical information, as well as operative and postoperative data. Patients were divided into two groups: those with acute appendicitis who underwent laparoscopic appendectomy ( = 46) and those without appendicitis ( = 46). The total white blood cell (WBC) count, percent of neutrophils, C-reactive protein (CRP) level, and salivary LRG1 were compared between groups. A commercially available enzyme-linked immunosorbent assay (ELISA) LRG kit was used to measure the LRG levels. The median salivary LRG1 level was significantly higher in the group of children with pathohistologically confirmed acute appendicitis compared to the control group: 233.45 ng/mL (IQR 114.9, 531.2) vs. 55.95 ng/mL (IQR 51.5, 117.9), < 0.001. LRG1 had an overall good receiver-operator characteristic area under the curve of 0.85 (95% CI 0.76-0.92; < 0.001). The optimal LRG1 cutoff with best separation between acute appendicitis and the controls was >352.6 ng/mL (95% CI from >270.7 to >352.6). Although the specificity was 100% at this cutoff, the sensitivity for identifying appendicitis was 36%. In addition, a significant difference was found between groups in the laboratory values of all inflammatory markers tested: WBC, absolute neutrophil count, and CRP ( < 0.001 for all). Although LRG1 in saliva showed a good AUC parameter and significantly higher values in patients with acute appendicitis compared to the controls, its usefulness in the patient population who present at emergency departments with abdominal pain is debatable. Future studies should focus on investigating its diagnostic potential.