The Drosophila Post-mating Response: Gene Expression and Behavioral Changes Reveal Perdurance and Variation in Cross-Tissue Interactions

Abstract Examining cross-tissue interactions is important for understanding physiology and homeostasis. In animals, the female gonad produces signaling molecules that act distally. We examine gene expression in Drosophila melanogaster female head tissues in 1) virgins without a germline compared to...

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Bibliographic Details
Published in:G3 : genes - genomes - genetics 2020-03, Vol.10 (3), p.967-983
Main Authors: Newell, Nicole R, Ray, Surjyendu, Dalton, Justin E, Fortier, Julia C, Kao, Joyce Y, Chang, Peter L, Nuzhdin, Sergey V, Arbeitman, Michelle N
Format: Article
Language:English
Subjects:
Animals
cross-tissue interactions
drosophila
Drosophila melanogaster - genetics
Drosophila melanogaster - physiology
Female
Fertility - genetics
Gene Expression
Genes, Insect
Genetics of Sex
Genome-Wide Association Study
Genotype
gwas
INDEL Mutation
Male
Polymorphism, Genetic
post-mating
Reproduction - genetics
rna-seq
Sexual Behavior, Animal
sleep
Wnt Signaling Pathway - genetics
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Summary:Abstract Examining cross-tissue interactions is important for understanding physiology and homeostasis. In animals, the female gonad produces signaling molecules that act distally. We examine gene expression in Drosophila melanogaster female head tissues in 1) virgins without a germline compared to virgins with a germline, 2) post-mated females with and without a germline compared to virgins, and 3) post-mated females mated to males with and without a germline compared to virgins. In virgins, the absence of a female germline results in expression changes in genes with known roles in nutrient homeostasis. At one- and three-day(s) post-mating, genes that change expression are enriched with those that function in metabolic pathways, in all conditions. We systematically examine female post-mating impacts on sleep, food preference and re-mating, in the strains and time points used for gene expression analyses and compare to published studies. We show that post-mating, gene expression changes vary by strain, prompting us to examine variation in female re-mating. We perform a genome-wide association study that identifies several DNA polymorphisms, including four in/near Wnt signaling pathway genes. Together, these data reveal how gene expression and behavior in females are influenced by cross-tissue interactions, by examining the impact of mating, fertility, and genotype.
ISSN:2160-1836
2160-1836
DOI:10.1534/g3.119.400963