Alteration of rhesus macaque serum N-glycome during infection with the human parasitic filarial nematode Brugia malayi

Serum N-glycan profiling studies during the past decades have shown robust associations between N-glycan changes and various biological conditions, including infections, in humans. Similar studies are scarcer for other mammals, despite the tremendous potential of serum N-glycans as biomarkers for in...

Full description

Saved in:
Bibliographic Details
Published in:Scientific reports 2022-09, Vol.12 (1), p.15763-15763, Article 15763
Main Authors: Petralia, Laudine M. C., Santha, Esrath, Behrens, Anna-Janina, Nguyen, D. Linh, Ganatra, Mehul B., Taron, Christopher H., Khatri, Vishal, Kalyanasundaram, Ramaswamy, van Diepen, Angela, Hokke, Cornelis H., Foster, Jeremy M.
Format: Article
Language:English
Subjects:
631/1647/296
631/250/255
631/337/458/1524
631/45/1268
631/45/72
631/92/221
631/92/458
692/53/2421
692/699/255
Animal diseases
Animal models
Brugia malayi
Filariasis
Humanities and Social Sciences
Immune response
Immunoglobulin G
Infections
Infectious diseases
Macaca mulatta
Mammals
multidisciplinary
N-glycans
Polysaccharides
Science
Science (multidisciplinary)
Vector-borne diseases
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Serum N-glycan profiling studies during the past decades have shown robust associations between N-glycan changes and various biological conditions, including infections, in humans. Similar studies are scarcer for other mammals, despite the tremendous potential of serum N-glycans as biomarkers for infectious diseases in animal models of human disease and in the veterinary context. To expand the knowledge of serum N-glycan profiles in important mammalian model systems, in this study, we combined MALDI-TOF-MS analysis and HILIC-UPLC profiling of released N-glycans together with glycosidase treatments to characterize the glycan structures present in rhesus macaque serum. We used this baseline to monitor changes in serum N-glycans during infection with Brugia malayi , a parasitic nematode of humans responsible for lymphatic filariasis, in a longitudinal cohort of infected rhesus macaques. Alterations of the HILIC-UPLC profile, notably of abundant structures, became evident as early as 5 weeks post-infection. Given its prominent role in the immune response, contribution of immunoglobulin G to serum N-glycans was investigated. Finally, comparison with similar N-glycan profiling performed during infection with the dog heartworm Dirofilaria immitis suggests that many changes observed in rhesus macaque serum N-glycans are specific for lymphatic filariasis.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-022-19964-1