Artificial Neural Networks Elucidated the Essential Role of Mineral Nutrients versus Vitamins and Plant Growth Regulators in Achieving Healthy Micropropagated Plants

The design of an adequate culture medium is an essential step in the micropropagation process of plant species. Adjustment and balance of medium components involve the interaction of several factors, such as mineral nutrients, vitamins, and plant growth regulators (PGRs). This work aimed to shed lig...

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Bibliographic Details
Published in:Plants (Basel) 2022-05, Vol.11 (10), p.1284
Main Authors: Arteta, Tomás A, Hameg, Radhia, Landin, Mariana, Gallego, Pedro P, Barreal, M Esther
Format: Article
Language:English
Subjects:
Algorithms
Ammonium nitrate
Artificial intelligence
Artificial neural networks
basal medium composition
Benzyladenine
Culture media
Data mining
Design of experiments
Experimental design
Gibberellic acid
Growth regulators
in vitro culture medium
Inositol
Inositols
Micropropagation
mineral nutrition
Minerals
modeling
Neural networks
neurofuzzy logic
Nicotinic acid
Nutrients
Plant growth
Plant species
Pyridoxine
Quality assessment
Statistical analysis
Thiamine
Variables
Vitamin E
Vitamins
Woody plants
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Summary:The design of an adequate culture medium is an essential step in the micropropagation process of plant species. Adjustment and balance of medium components involve the interaction of several factors, such as mineral nutrients, vitamins, and plant growth regulators (PGRs). This work aimed to shed light on the role of these three components on the plant growth and quality of micropropagated woody plants, using as a plant model. Two experiments using a five-dimensional experimental design space were defined using the Design of Experiments (DoE) method, to study the effect of five mineral factors (NH NO , KNO , Mesos, Micros, and Iron) and five vitamins (Myo-inositol, thiamine, nicotinic acid, pyridoxine, and vitamin E). A third experiment, using 20 combinations of two PGRs: BAP (6-benzylaminopurine) and GA (gibberellic acid) was performed. Artificial Neural Networks (ANNs) algorithms were used to build models with the whole database to determine the effect of those components on several growth and quality parameters. Neurofuzzy logic allowed us to decipher and generate new knowledge on the hierarchy of some minerals as essential components of the culture media over vitamins and PRGs, suggesting rules about how MS basal media formulation could be modified to assess the quality of micropropagated woody plants.
ISSN:2223-7747
2223-7747
DOI:10.3390/plants11101284