The effect of CHRNA3 rs1051730 C>T and ABCB1 rs3842 A>G polymorphisms on non-small cell lung cancer and nicotine dependence in Iranian population

• Published in: Heliyon 2021-09, Vol.7 (9), p.e07867-e07867, Article e07867
• Main Authors: Veiskarami, Parisa, Houshmand, Massoud, Seifi, Sharareh, Ansarinejad, Nafiseh, Fardad, Farshid, Abbasi, Bahareh
• Format: Article
• Language: English
• Subjects: ABCB1 rs3842 A>G; CHRNA3 rs1051730 C>T; Lung cancer; Nicotine dependence; Polymorphism
• ISSN: 2405-8440; 2405-8440
• DOI: 10.1016/j.heliyon.2021.e07867

Lung cancer is still the leading cause of cancer mortality in all over the world. Nicotine and its derivatives are the most well-known carcinogens that participate in both etiology and progression of lung cancer. The objective of the current study was to investigate whether single nucleotide polymorphisms (SNPs) rs1051730C > T in CHRNA3 and rs3842A > G in ABCB1, two genes contributing in the mechanism of disposition and metabolism of nicotine and its derivatives, could modify the risk of developing lung cancer, as well as nicotine dependence in Iranian. The genotyping analysis for these two SNPs was conducted in a case-control study of 108 lung cancer cases and 120 healthy controls using ARMS-PCR and Tetra-primer ARMS-PCR techniques. The correlation between studied SNPs and lung cancer was assessed by the regression analysis. We observed a significant association between lung cancer and rs1051730C > T by using four genetic models: allele (OR:1.83; 95% CI:1.24–2.6; p = 0.002), dominant (OR: 2.19; 95% CI:1.27–3.78; p = 0.005), recessive (OR: 2.25; 95% CI: 1.02–4.95; p = 0.043) and additive (TT vs CC: OR:3.25; 95% CI:1.38–7.60; p = 0.007, CT vs CC: OR:1.96; 95% CI:1.10–3.48; p = 0.021). Furthermore, a significant association between this variant and nicotine dependence (OR: 2.27; 95% CI: 1.52–3.39; p = 0.00005) was reported. However, no association was found for rs3842A > G. The results suggested that the CHRNA3 rs1051730C > T via a smoking-dependent manner could modify susceptibility to lung cancer among Iranian population. Lung cancer; Nicotine dependence; Polymorphism; CHRNA3 rs1051730 C>T; ABCB1 rs3842 A>G