MWNTs or PEG as Stability Enhancers for DNA–Cationic Surfactant Gel Particles

Cationic surfactants interact with DNA (Deoxyribonucleic acid), forming surfactant-DNA complexes that offer particularly efficient control for encapsulation and release of DNA from DNA gel particles. In the present work, DNA-based particles were prepared using CTAB (Cetyltrimethylammonium bromide) a...

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Bibliographic Details
Published in:International journal of molecular sciences 2021-08, Vol.22 (16), p.8801
Main Authors: Mezei, Amalia, Pons, Ramon
Format: Article
Language:English
Subjects:
Additives
Biosensors
Carbon
Cations
composite
Controlled release
Cosmetics
Dehydration
Deoxyribonucleic acid
DNA
Drug delivery
Drug delivery systems
Gene therapy
multi-walled carbon nanotubes
Particulate composites
PEG
Pharmaceuticals
Polyethylene glycol
Rehydration
release
Solubilization
Stability
Surfactants
Sustained release
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Summary:Cationic surfactants interact with DNA (Deoxyribonucleic acid), forming surfactant-DNA complexes that offer particularly efficient control for encapsulation and release of DNA from DNA gel particles. In the present work, DNA-based particles were prepared using CTAB (Cetyltrimethylammonium bromide) as the cationic surfactant and modified using two different additives: (Multi-Walled Carbon Nanotubes) MWNT or PEG (Poly Ethylene Glycol). The use of both additives to form composites increased the stability of the gel particles. The stability was monitored by the release of DNA and CTAB in different pH solutions. However, not much is known about the influence of pH on DNA–surfactant interaction and the release of DNA and surfactant from gel particles. It was observed that the solubilization of DNA occurs only in very acid media, while that of CTAB does not depend on pH and gets to a plateau after about 8 h. Within 2 h in contact with a pH = 2 solution, about 1% DNA and CTAB was released. Complete destruction for the gel particles was observed in pH = 2 solution after 17 days for PEG and 20 days for MWNT. The composite particles show a considerably enlarged sustained release span compared to the unmodified ones. The dehydration-rehydration studies show that the structure of the composite gel particles, as determined from SAXS (Small-Angle-X-Ray-Scattering) experiments, is similar to that of the unmodified ones. These studies will allow a better knowledge of these particles’ formation and evolution in view of possible applications in drug delivery and release.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms22168801