The C-Terminal Repeat Units of SpaA Mediate Adhesion of Erysipelothrix rhusiopathiae to Host Cells and Regulate Its Virulence

Erysipelothrix rhusiopathiae is a causative agent of erysipelas in animals and erysipeloid in humans. However, current information regarding E. rhusiopathiae pathogenesis remains limited. Previously, we identified two E. rhusiopathiae strains, SE38 and G4T10, which were virulent and avirulent in pig...

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Published in:Biology (Basel, Switzerland) Switzerland), 2022-07, Vol.11 (7), p.1010
Main Authors: Wu, Chao, Zhang, Zhewen, Kang, Chao, Zhang, Qiang, Zhu, Weifeng, Zhang, Yadong, Zhang, Hao, Xiao, Jingfa, Jin, Meilin
Format: Article
Language:English
Subjects:
Antigens
C-terminal repeat
comparative genomics
Endothelial cells
Erysipelas
Erysipeloid
Erysipelothrix rhusiopathiae
Gene expression
Genomes
Genomic analysis
Genomics
Hogs
Homologous recombination
Infections
Mutants
Pathogenesis
Pathogens
Phylogenetics
Plasmids
Proteins
Reconciliation
SpaA
SpaA gene
Trees
Virulence
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Summary:Erysipelothrix rhusiopathiae is a causative agent of erysipelas in animals and erysipeloid in humans. However, current information regarding E. rhusiopathiae pathogenesis remains limited. Previously, we identified two E. rhusiopathiae strains, SE38 and G4T10, which were virulent and avirulent in pigs, respectively. Here, to further study the pathogenic mechanism of E. rhusiopathiae, we sequenced and assembled the genomes of strains SE38 and G4T10, and performed a comparative genomic analysis to identify differences or mutations in virulence-associated genes. Next, we comparatively analyzed 25 E. rhusiopathiae virulence-associated genes in SE38 and G4T10. Compared with that of SE38, the spaA gene of the G4T10 strain lacked 120 bp, encoding repeat units at the C-terminal of SpaA. To examine whether these deletions or splits influence E. rhusiopathiae virulence, these 120 bp were successfully deleted from the spaA gene in strain SE38 by homologous recombination. The mutant strain ΔspaA displayed attenuated virulence in mice and decreased adhesion to porcine iliac artery endothelial cells, which was also observed using the corresponding mutant protein SpaA’. Our results demonstrate that SpaA-mediated adhesion between E. rhusiopathiae and host cells is dependent on its C-terminal repeat units.
ISSN:2079-7737
2079-7737
DOI:10.3390/biology11071010