Long Noncoding RNA OIP5-AS1 Promotes the Progression of Liver Hepatocellular Carcinoma via Regulating the hsa-miR-26a-3p/EPHA2 Axis

• Published in: Molecular therapy. Nucleic acids 2020-09, Vol.21, p.229-241
• Main Authors: Ma, Yu-Shui, Chu, Kai-Jian, Ling, Chang-Chun, Wu, Ting-Miao, Zhu, Xu-Chao, Liu, Ji-Bin, Yu, Fei, Li, Zhi-Zhen, Wang, Jing-Han, Gao, Qing-Xiang, Yi, Bin, Wang, Hui-Min, Gu, Li-Peng, Li, Liu, Tian, Lin-Lin, Shi, Yi, Jiang, Xiao-Qing, Fu, Da, Zhang, Xiong-Wen
• Format: Article
• Language: English
• Subjects: EPHA2; hsa-miR-26a-3p; LIHC; OIP5-AS1; prognosis
• ISSN: 2162-2531; 2162-2531
• DOI: 10.1016/j.omtn.2020.05.032

Numerous studies have suggested that dysregulated long noncoding RNAs (lncRNAs) contributed to the development and progression of many cancers. lncRNA OIP5 antisense RNA 1 (OIP5-AS1) has been reported to be increased in several cancers. However, the roles of OIP5-AS1 in liver hepatocellular carcinoma (LIHC) remain to be investigated. In this study, we demonstrated that OIP5-AS1 was upregulated in LIHC tissue specimens and its overexpression was associated with the poor survival of patients with LIHC. Furthermore, loss-of function experiments indicated that OIP5-AS1 promoted cell proliferation and inhibited cell apoptosis both in vitro and in vivo. Moreover, binding sites between OIP5-AS1 and hsa-miR-26a-3p as well as between hsa-miR-26a-3p and EPHA2 were confirmed by luciferase assays. Finally, a rescue assay was performed to prove the effect of the OIP5-AS1/hsa-miR-26a-3p/EPHA2 axis on LIHC cell biological behaviors. Based on all of the above findings, our results suggested that OIP5-AS1 promoted LIHC cell proliferation and invasion via regulating the hsa-miR-26a-3p/EPHA2 axis.