Structural and functional analyses of the echinomycin resistance conferring protein Ecm16 from Streptomyces lasalocidi

Echinomycin is a natural product DNA bisintercalator antibiotic. The echinomycin biosynthetic gene cluster in Streptomyces lasalocidi includes a gene encoding the self-resistance protein Ecm16. Here, we present the 2.0 Å resolution crystal structure of Ecm16 bound to adenosine diphosphate. The struc...

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Bibliographic Details
Published in:Scientific reports 2023-05, Vol.13 (1), p.7980-7980, Article 7980
Main Authors: Gade, Priyanka, Erlandson, Amanda, Ullah, Anwar, Chen, Xi, Mathews, Irimpan I., Mera, Paola E., Kim, Chu-Young
Format: Article
Language:English
Subjects:
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631/45
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Adenosine diphosphate
Adenosine Triphosphatases - metabolism
Amino acid sequence
Amino acids
Anti-Bacterial Agents - chemistry
Antibiotics
BASIC BIOLOGICAL SCIENCES
biochemistry
Crystal structure
Deoxyribonucleic acid
DNA
DNA - metabolism
DNA damage
Echinomycin
Echinomycin - pharmacology
Humanities and Social Sciences
microbiology
multidisciplinary
Mutagenesis
Natural products
Nucleotide excision repair
Nucleotide sequence
Science
Science (multidisciplinary)
Streptomyces
Streptomyces - genetics
Streptomyces - metabolism
structural biology
Structure-function relationships
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Summary:Echinomycin is a natural product DNA bisintercalator antibiotic. The echinomycin biosynthetic gene cluster in Streptomyces lasalocidi includes a gene encoding the self-resistance protein Ecm16. Here, we present the 2.0 Å resolution crystal structure of Ecm16 bound to adenosine diphosphate. The structure of Ecm16 closely resembles that of UvrA, the DNA damage sensor component of the prokaryotic nucleotide excision repair system, but Ecm16 lacks the UvrB-binding domain and its associated zinc-binding module found in UvrA. Mutagenesis study revealed that the insertion domain of Ecm16 is required for DNA binding. Furthermore, the specific amino acid sequence of the insertion domain allows Ecm16 to distinguish echinomycin-bound DNA from normal DNA and link substrate binding to ATP hydrolysis activity. Expression of ecm16 in the heterologous host Brevibacillus choshinensis conferred resistance against echinomycin and other quinomycin antibiotics, including thiocoraline, quinaldopeptin, and sandramycin. Our study provides new insight into how the producers of DNA bisintercalator antibiotics fend off the toxic compounds that they produce.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-023-34437-9