WDR90 is a centriolar microtubule wall protein important for centriole architecture integrity

Centrioles are characterized by a nine-fold arrangement of microtubule triplets held together by an inner protein scaffold. These structurally robust organelles experience strenuous cellular processes such as cell division or ciliary beating while performing their function. However, the molecular me...

Full description

Saved in:
Bibliographic Details
Published in:eLife 2020-09, Vol.9
Main Authors: Steib, Emmanuelle, Laporte, Marine H, Gambarotto, Davide, Olieric, Natacha, Zheng, Celine, Borgers, Susanne, Olieric, Vincent, Le Guennec, Maeva, Koll, France, Tassin, Anne-Marie, Steinmetz, Michel O, Guichard, Paul, Hamel, Virginie
Format: Article
Language:English
Subjects:
Animals
Cattle
Cell Biology
Cell Line
Cells (Biology)
Cells, Cultured
centriole
Centrioles - metabolism
Centrioles - ultrastructure
Chlamydomonas
Chromatin
Cytoskeletal Proteins - genetics
Cytoskeletal Proteins - metabolism
Cytoskeletal Proteins - ultrastructure
expansion microscopy
Humans
inner scaffold
Life Sciences
microtubule
Microtubules - metabolism
Microtubules - ultrastructure
Plant Proteins - genetics
Plant Proteins - metabolism
Plant Proteins - ultrastructure
Protein binding
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Centrioles are characterized by a nine-fold arrangement of microtubule triplets held together by an inner protein scaffold. These structurally robust organelles experience strenuous cellular processes such as cell division or ciliary beating while performing their function. However, the molecular mechanisms underlying the stability of microtubule triplets, as well as centriole architectural integrity remain poorly understood. Here, using ultrastructure expansion microscopy for nanoscale protein mapping, we reveal that POC16 and its human homolog WDR90 are components of the microtubule wall along the central core region of the centriole. We further found that WDR90 is an evolutionary microtubule associated protein. Finally, we demonstrate that WDR90 depletion impairs the localization of inner scaffold components, leading to centriole structural abnormalities in human cells. Altogether, this work highlights that WDR90 is an evolutionary conserved molecular player participating in centriole architecture integrity.
ISSN:2050-084X
2050-084X
DOI:10.7554/ELIFE.57205