Different Transcriptional Profiles of Human Monocyte-Derived Dendritic Cells Infected with Distinct Strains of Mycobacterium tuberculosis and Mycobacterium bovis Bacillus Calmette-Guérin

In order to analyze dendritic cells (DCs) activation following infection with different mycobacterial strains, we studied the expression profiles of 165 genes of human monocyte-derived DCs infected with H37Rv, a virulent Mycobacterium tuberculosis (MTB) laboratory strain, CMT97, a clinical MTB isola...

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Published in:Clinical & developmental immunology 2011-01, Vol.2011 (2011), p.1-14
Main Authors: Sanarico, Nunzia, Colone, Alessia, Grassi, Manuela, Speranza, Viviana, Giovannini, Daniela, Ciaramella, Antonio, Colizzi, Vittorio, Mariani, Francesca
Format: Article
Language:English
Subjects:
Bacteria
Dendritic Cells - immunology
Dendritic Cells - metabolism
Dendritic Cells - microbiology
Dendritic Cells - pathology
Gene expression
Gene Expression Profiling
Humans
Immune system
Immunology
Immunophenotyping
Interleukin-10 - genetics
Interleukin-10 - metabolism
Interleukins - genetics
Interleukins - metabolism
Minor Histocompatibility Antigens
Monocytes - pathology
Mycobacterium bovis
Mycobacterium bovis - immunology
Mycobacterium bovis - pathogenicity
Mycobacterium tuberculosis
Mycobacterium tuberculosis - immunology
Mycobacterium tuberculosis - pathogenicity
Neurosciences
Receptors, CCR7 - genetics
Receptors, CCR7 - metabolism
Species Specificity
Studies
Tuberculosis - immunology
Tuberculosis - microbiology
Tuberculosis - prevention & control
Tuberculosis Vaccines
Tumor Necrosis Factor-alpha - genetics
Tumor Necrosis Factor-alpha - metabolism
Virulence
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description In order to analyze dendritic cells (DCs) activation following infection with different mycobacterial strains, we studied the expression profiles of 165 genes of human monocyte-derived DCs infected with H37Rv, a virulent Mycobacterium tuberculosis (MTB) laboratory strain, CMT97, a clinical MTB isolate, Mycobacterium bovis bacillus Calmette-Guérin (BCG), Aventis Pasteur, and BCG Japan, both employed as vaccine against tuberculosis. The analysis of the gene expression reveals that, despite a set of genes similarly modulated, DCs response resulted strain dependent. In particular, H37Rv significantly upregulated EBI3 expression compared with BCG Japan, while it was the only strain that failed to release a significant IL-10 amount. Of note, BCG Japan showed a marked increase in CCR7 and TNF-α expression regarding both MTB strains and it resulted the only strain failing in exponential intracellular growth. Our results suggest that DCs display the ability to elicit a tailored strain-specific immune response.
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The analysis of the gene expression reveals that, despite a set of genes similarly modulated, DCs response resulted strain dependent. In particular, H37Rv significantly upregulated EBI3 expression compared with BCG Japan, while it was the only strain that failed to release a significant IL-10 amount. Of note, BCG Japan showed a marked increase in CCR7 and TNF-α expression regarding both MTB strains and it resulted the only strain failing in exponential intracellular growth. 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subjects Bacteria
Dendritic Cells - immunology
Dendritic Cells - metabolism
Dendritic Cells - microbiology
Dendritic Cells - pathology
Gene expression
Gene Expression Profiling
Humans
Immune system
Immunology
Immunophenotyping
Interleukin-10 - genetics
Interleukin-10 - metabolism
Interleukins - genetics
Interleukins - metabolism
Minor Histocompatibility Antigens
Monocytes - pathology
Mycobacterium bovis
Mycobacterium bovis - immunology
Mycobacterium bovis - pathogenicity
Mycobacterium tuberculosis
Mycobacterium tuberculosis - immunology
Mycobacterium tuberculosis - pathogenicity
Neurosciences
Receptors, CCR7 - genetics
Receptors, CCR7 - metabolism
Species Specificity
Studies
Tuberculosis - immunology
Tuberculosis - microbiology
Tuberculosis - prevention & control
Tuberculosis Vaccines
Tumor Necrosis Factor-alpha - genetics
Tumor Necrosis Factor-alpha - metabolism
Virulence
title Different Transcriptional Profiles of Human Monocyte-Derived Dendritic Cells Infected with Distinct Strains of Mycobacterium tuberculosis and Mycobacterium bovis Bacillus Calmette-Guérin
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