Target of Rapamycin Signaling Involved in the Regulation of Photosynthesis and Cellular Metabolism in Chlorella sorokiniana

Target of rapamycin (TOR) is a serine/threonine protein kinase that plays a central regulating role in cell proliferation, growth, and metabolism, but little is known about the TOR signaling pathway in Chlorella sorokiniana. In this study, a Chlorella sorokiniana DP-1 strain was isolated and identif...

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Bibliographic Details
Published in:International journal of molecular sciences 2022-07, Vol.23 (13), p.7451
Main Authors: Li, Linxuan, Zhu, Tingting, Huang, Lele, Ren, Maozhi
Format: Article
Language:English
Subjects:
Algae
Autophagy
AZD8055
Carbon fixation
Cell growth
Cell proliferation
Chlorella
Chlorella sorokiniana
Chlorophyll
Conserved sequence
Genomes
Homology
Kinases
Lipids
Nucleotide sequence
Photosynthesis
Protein biosynthesis
Protein kinase
Protein synthesis
Protein-serine/threonine kinase
Proteins
Rapamycin
Sequence analysis
Signal processing
Signal transduction
target of rapamycin
TOR protein
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Summary:Target of rapamycin (TOR) is a serine/threonine protein kinase that plays a central regulating role in cell proliferation, growth, and metabolism, but little is known about the TOR signaling pathway in Chlorella sorokiniana. In this study, a Chlorella sorokiniana DP-1 strain was isolated and identified, and its nutritional compositions were analyzed. Based on homologous sequence analysis, the conserved CsTOR protein was found in the genome of Chlorella sorokiniana. In addition, the key components of TOR complex 1 (TORC1) were present, but the components of TORC2 (RICTOR and SIN1) were absent in Chlorella sorokiniana. Pharmacological assays showed that Chlorella sorokiniana DP-1 was insensitive to rapamycin, Torin1 and KU0063794, whereas AZD8055 could significantly inhibit the growth of Chlorella sorokiniana. RNA-seq analysis showed that CsTOR regulated various metabolic processes and signal transduction pathways in AZD8055-treated Chlorella sorokiniana DP-1. Most genes involved in photosynthesis and carbon fixation in Chlorella sorokiniana DP-1 were significantly downregulated under CsTOR inhibition, indicating that CsTOR positively regulated the photosynthesis in Chlorella sorokiniana. Furthermore, CsTOR controlled protein synthesis and degradation by positively regulating ribosome synthesis and negatively regulating autophagy. These observations suggested that CsTOR plays an important role in photosynthesis and cellular metabolism, and provide new insights into the function of CsTOR in Chlorella sorokiniana.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms23137451