Microbial diversity and metabolic pathways linked to benzene degradation in petrochemical-polluted groundwater

• Published in: Environment international 2024-06, Vol.188, p.108755-108755, Article 108755
• Main Authors: Zhang, Ruihuan, Ye, Zhencheng, Guo, Xue, Yang, Yunfeng, Li, Guanghe
• Format: Article
• Language: English
• Subjects: Benzene degradation; Groundwater; Metagenome binning; Microbial diversity; Petrochemical pollution
• ISSN: 0160-4120; 1873-6750
• DOI: 10.1016/j.envint.2024.108755

•Proteobacteria was the predominant phylum in groundwater at the petrochemical site.•The concentration of TPH (C10-C40) was negatively correlated with the α-diversity of microbial communities in groundwater.•A staggering 80% of anaerobic benzene-degrading Metagenome-Assembled Genomes (MAGs) were taxonomically unclassified.•Aerobic degradation of benzene predominantly occurred through monooxygenation in groundwater.•Deep sequencing and high-quality MAGs provide a high-resolution approach to groundwater monitoring. The rapid advance in shotgun metagenome sequencing has enabled us to identify uncultivated functional microorganisms in polluted environments. While aerobic petrochemical-degrading pathways have been extensively studied, the anaerobic mechanisms remain less explored. Here, we conducted a study at a petrochemical-polluted groundwater site in Henan Province, Central China. A total of twelve groundwater monitoring wells were installed to collect groundwater samples. Benzene appeared to be the predominant pollutant, detected in 10 out of 12 samples, with concentrations ranging from 1.4 μg/L to 5,280 μg/L. Due to the low aquifer permeability, pollutant migration occurred slowly, resulting in relatively low benzene concentrations downstream within the heavily polluted area. Deep metagenome sequencing revealed Proteobacteria as the dominant phylum, accounting for over 63 % of total abundances. Microbial α-diversity was low in heavily polluted samples, with community compositions substantially differing from those in lightly polluted samples. dmpK encoding the phenol/toluene 2-monooxygenase was detected across all samples, while the dioxygenase bedC1 was not detected, suggesting that aerobic benzene degradation might occur through monooxygenation. Sequence assembly and binning yielded 350 high-quality metagenome-assembled genomes (MAGs), with 30 MAGs harboring functional genes associated with aerobic or anaerobic benzene degradation. About 80 % of MAGs harboring functional genes associated with anaerobic benzene degradation remained taxonomically unclassified at the genus level, suggesting that our current database coverage of anaerobic benzene-degrading microorganisms is very limited. Furthermore, two genes integral to anaerobic benzene metabolism, i.e, benzoyl-CoA reductase (bamB) and glutaryl-CoA dehydrogenase (acd), were not annotated by metagenome functional analyses but were identified within the MAGs, signifying the importance of integrating both c