Thermodynamic Modeling of Solvent-Assisted Lipid Bilayer Formation Process

The solvent-assisted lipid bilayer (SALB) formation method provides a simple and efficient, microfluidic-based strategy to fabricate supported lipid bilayers (SLBs) with rich compositional diversity on a wide range of solid supports. While various studies have been performed to characterize SLBs for...

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Bibliographic Details
Published in:Micromachines (Basel) 2022-01, Vol.13 (1), p.134
Main Authors: Xu, Hongmei, Tae, Hyunhyuk, Cho, Nam-Joon, Huang, Changjin, Hsia, K Jimmy
Format: Article
Language:English
Subjects:
Energy
Experiments
Hydration
Lipids
Microfluidics
Modelling
Parameter identification
Phase transitions
SALB
self-assembly
Silica
solvent exchange
Solvents
Substrates
supported lipid bilayer
Surface properties
Thermodynamic models
thermodynamics
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Summary:The solvent-assisted lipid bilayer (SALB) formation method provides a simple and efficient, microfluidic-based strategy to fabricate supported lipid bilayers (SLBs) with rich compositional diversity on a wide range of solid supports. While various studies have been performed to characterize SLBs formed using the SALB method, relatively limited work has been carried out to understand the underlying mechanisms of SALB formation under various experimental conditions. Through thermodynamic modeling, we studied the experimental parameters that affect the SALB formation process, including substrate surface properties, initial lipid concentration, and temperature. It was found that all the parameters are critically important to successfully form high-quality SLBs. The model also helps to identify the range of parameter space within which conformal, homogeneous SLBs can be fabricated, and provides mechanistic guidance to optimize experimental conditions for lipid membrane-related applications.
ISSN:2072-666X
2072-666X
DOI:10.3390/mi13010134