Integrative Bone Metabolomics—Lipidomics Strategy for Pathological Mechanism of Postmenopausal Osteoporosis Mouse Model

Osteoporosis, characterized by bone mass reduction and increased fractures, has become a global health problem that seriously affects the health of people, especially postmenopausal women; however, the current pathogenesis of postmenopausal osteoporosis (PMOP) has not been thoroughly elucidated to d...

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Bibliographic Details
Published in:Scientific reports 2018-11, Vol.8 (1), p.16456-11, Article 16456
Main Authors: Zhao, Hongxia, Li, Xiaoqun, Zhang, Dianying, Chen, Haiyan, Chao, Yufan, Wu, Kaiwen, Dong, Xin, Su, Jiacan
Format: Article
Language:English
Subjects:
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Amino acids
Bone growth
Bone mass
Bone resorption
Bone Volume/total Volume (BV/TV)
Femur
Fractures
Global health
Glycerophospholipids
Humanities and Social Sciences
Lipid metabolism
Lipidomic Analysis
Lipids
Metabolic disorders
Metabolism
Metabolites
Metabolomics
multidisciplinary
Osteoporosis
Osteoporosis Mice
Ovariectomy
Post-menopause
Postmenopausal Osteoporosis
Science
Science (multidisciplinary)
Sphingolipids
Sterols
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Summary:Osteoporosis, characterized by bone mass reduction and increased fractures, has become a global health problem that seriously affects the health of people, especially postmenopausal women; however, the current pathogenesis of postmenopausal osteoporosis (PMOP) has not been thoroughly elucidated to date. In this study, bilateral ovariectomy was performed to establish an OVX mouse model of osteoporosis. UPLC-Q-TOF-MS-based lipidomics in combination with metabolomics were used to analyze the femur tissue of osteoporosis mice. We found that 11 polar metabolites and 93 lipid metabolites were significantly changed and were involved in amino acid metabolism, nucleotide metabolism and lipid metabolism. Among the lipids, fatty acyls, glycerolipids, glycerophospholipids, sphingolipids and sterols showed robust changes. These results revealed that several metabolic disorders caused by changes in the hormone levels in OVX, especially disordered lipid metabolism, are closely related to the imbalance between bone resorption and formation and may underlie the development of PMOP. The data generated via lipidomics and metabolomics presented in this study shows good applicability and wide coverage in the construction of the metabolic profile of bone tissue. Therefore, this approach may provide the pathway focusing and data support at the metabolite level for the in-depth mechanism of PMOP.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-018-34574-6