The Effect of Local Anesthetics on Neutrophils in the Context of Different Isolation Techniques

Various functions of polymorphonuclear neutrophils (PMNs) are related to diseases and postoperative plasma changes. The influence of some local anesthetics (LAs) on PMNs obtained by conventional isolation methods and their functions has already been demonstrated. This study investigates the effect o...

Full description

Saved in:
Bibliographic Details
Published in:Biomedicines 2023-08, Vol.11 (8), p.2170
Main Authors: Sixt, Sara, Gruber, Michael, Kolle, Gesche, Galla, Thies, Bitzinger, Diane
Format: Article
Language:English
Subjects:
Anesthetics
Antigens
Bupivacaine
Centrifugation
Chemotaxis
Flow cytometry
Granulocytes
Immune system
isolation
Leukocyte migration
Leukocytes (neutrophilic)
Leukocytes (polymorphonuclear)
Lidocaine
Life sciences
Lithium
Local anesthetics
Lysis
Methods
Neutrophils
Pathogens
PMN functions
PMNs
Ropivacaine
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Various functions of polymorphonuclear neutrophils (PMNs) are related to diseases and postoperative plasma changes. The influence of some local anesthetics (LAs) on PMNs obtained by conventional isolation methods and their functions has already been demonstrated. This study investigates the effect of selected LAs on PMNs, comparing a new isolation method with conventional ones. To obtain the PMNs, we performed either gelafundin sedimentation, hypotonic lysis or density gradient centrifugation. Subsequently, PMNs were mixed with different concentrations of bupivacaine, levobupivacaine, lidocaine or ropivacaine. Live cell imaging and flow cytometry were performed to quantify the migration, ROS production, NETosis and antigen expression of PMNs. We found the inhibition of chemotaxis and ROS production by LAs. PMNs showed a strong reduction in time to half maximal NETosis in response to bupivacaine and lidocaine, but not to levobupivacaine and ropivacaine. We also found distinct differences in survival time and migration duration between the isolation methods. This suggests that the careful selection of LAs has a short-term impact on in vitro PMNs.
ISSN:2227-9059
2227-9059
DOI:10.3390/biomedicines11082170