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First Pregnancy, Somatic and Psychological Status of a 4-Year-Old Child Born following Annexin V TESA Sperm Separation
Abstract Introduction Sperm DNA integrity is a crucial paternal factor affecting fertilization and pregnancy rates, as well as embryo development. Case The present case report describes the successful pregnancy after testicular sperm aspiration (TESA) combined with intracytoplasmic sperm injection...
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Published in: | American journal of perinatology reports 2015-10, Vol.5 (2), p.e105-e108 |
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container_title | American journal of perinatology reports |
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creator | Lukaszuk, Krzysztof Wcislo, Monika Liss, Joanna Stachowicz, Anna Jakiel, Grzegorz Lukaszuk, Mariusz Pastuszek, Ewa Woclawek-Potocka, Izabela Galvao, Antonio Bialobrzeska, Dorota |
description | Abstract
Introduction
Sperm DNA integrity is a crucial paternal factor affecting fertilization and pregnancy rates, as well as embryo development.
Case
The present case report describes the successful pregnancy after testicular sperm aspiration (TESA) combined with intracytoplasmic sperm injection (ICSI) (TESA-ICSI) in a couple where the male presented high sperm DNA fragmentation. In order to sort damaged sperm presenting DNA fragmentation, magnetic activated cell sorting (MACS) with annexin V microbeads (MACS Miltenyi Biotec, Teterow, Germany) was used.
Conclusion
The authors present the first description of a successful medical case using TESA-ICSI annexin V sperm sorting. Additionally, a follow-up of the child at the age of 4 years old was done. |
doi_str_mv | 10.1055/s-0035-1548726 |
format | article |
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Introduction
Sperm DNA integrity is a crucial paternal factor affecting fertilization and pregnancy rates, as well as embryo development.
Case
The present case report describes the successful pregnancy after testicular sperm aspiration (TESA) combined with intracytoplasmic sperm injection (ICSI) (TESA-ICSI) in a couple where the male presented high sperm DNA fragmentation. In order to sort damaged sperm presenting DNA fragmentation, magnetic activated cell sorting (MACS) with annexin V microbeads (MACS Miltenyi Biotec, Teterow, Germany) was used.
Conclusion
The authors present the first description of a successful medical case using TESA-ICSI annexin V sperm sorting. Additionally, a follow-up of the child at the age of 4 years old was done.</description><identifier>ISSN: 2157-6998</identifier><identifier>EISSN: 2157-7005</identifier><identifier>DOI: 10.1055/s-0035-1548726</identifier><identifier>PMID: 26495164</identifier><language>eng</language><publisher>333 Seventh Avenue, New York, NY 10001, USA: Thieme Medical Publishers</publisher><subject>Case Report ; intracytoplasmic sperm injection ; sperm ; sperm dna integrity ; testicular sperm aspiration</subject><ispartof>American journal of perinatology reports, 2015-10, Vol.5 (2), p.e105-e108</ispartof><rights>Thieme Medical Publishers</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c391t-f48f973fe65cb0d2f9b5f94ad50741064828578818dbef07a19df15160c957a3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4603856/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4603856/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,20891,27924,27925,53791,53793,54587,54615</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26495164$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lukaszuk, Krzysztof</creatorcontrib><creatorcontrib>Wcislo, Monika</creatorcontrib><creatorcontrib>Liss, Joanna</creatorcontrib><creatorcontrib>Stachowicz, Anna</creatorcontrib><creatorcontrib>Jakiel, Grzegorz</creatorcontrib><creatorcontrib>Lukaszuk, Mariusz</creatorcontrib><creatorcontrib>Pastuszek, Ewa</creatorcontrib><creatorcontrib>Woclawek-Potocka, Izabela</creatorcontrib><creatorcontrib>Galvao, Antonio</creatorcontrib><creatorcontrib>Bialobrzeska, Dorota</creatorcontrib><title>First Pregnancy, Somatic and Psychological Status of a 4-Year-Old Child Born following Annexin V TESA Sperm Separation</title><title>American journal of perinatology reports</title><addtitle>AJP Rep</addtitle><description>Abstract
Introduction
Sperm DNA integrity is a crucial paternal factor affecting fertilization and pregnancy rates, as well as embryo development.
Case
The present case report describes the successful pregnancy after testicular sperm aspiration (TESA) combined with intracytoplasmic sperm injection (ICSI) (TESA-ICSI) in a couple where the male presented high sperm DNA fragmentation. In order to sort damaged sperm presenting DNA fragmentation, magnetic activated cell sorting (MACS) with annexin V microbeads (MACS Miltenyi Biotec, Teterow, Germany) was used.
Conclusion
The authors present the first description of a successful medical case using TESA-ICSI annexin V sperm sorting. Additionally, a follow-up of the child at the age of 4 years old was done.</description><subject>Case Report</subject><subject>intracytoplasmic sperm injection</subject><subject>sperm</subject><subject>sperm dna integrity</subject><subject>testicular sperm aspiration</subject><issn>2157-6998</issn><issn>2157-7005</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>0U6</sourceid><sourceid>DOA</sourceid><recordid>eNp1kU1r3DAQhkVoaUKaa49BP6BOJVuSpUthuyRpIJCAl0JPQtaHV4stLZI37f77KN1taA6dw4wYvfOI0QvAJ4yuMKL0S64QamiFKeFtzU7AWY1pW7UI0XfHMxOCn4KLnDeoBKGUYvwBnNaMCIoZOQNPNz7lGT4mOwQV9P4z7OKkZq-hCgY-5r1exzEOXqsRdrOadxlGBxUk1U-rUvUwGrhc-5K_xRSgi-MYf_kwwEUI9rcP8AdcXXcL2G1tmmBntyoVeAwfwXunxmwvjvUcrG6uV8vv1f3D7d1ycV_pRuC5coQ70TbOMqp7ZGoneuoEUYailmDECK85bTnH3PTWoVZhYRwumyEtaKuac3B3wJqoNnKb_KTSXkbl5Z9GTINUqSw7Wol5L7RhBjGrSaNxjxBWBNW0ZrYlvS6srwfWdtdP1mgb5qTGN9C3N8Gv5RCfJGGo4ZQVwNUBoFPMOVn3OouRfPFTZvnipzz6WQYu_33xVf7XvSKoDoJ57e1k5SbuUijf-T_gM2nMqSQ</recordid><startdate>20151001</startdate><enddate>20151001</enddate><creator>Lukaszuk, Krzysztof</creator><creator>Wcislo, Monika</creator><creator>Liss, Joanna</creator><creator>Stachowicz, Anna</creator><creator>Jakiel, Grzegorz</creator><creator>Lukaszuk, Mariusz</creator><creator>Pastuszek, Ewa</creator><creator>Woclawek-Potocka, Izabela</creator><creator>Galvao, Antonio</creator><creator>Bialobrzeska, Dorota</creator><general>Thieme Medical Publishers</general><general>Thieme Medical Publishers, Inc</general><scope>0U6</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20151001</creationdate><title>First Pregnancy, Somatic and Psychological Status of a 4-Year-Old Child Born following Annexin V TESA Sperm Separation</title><author>Lukaszuk, Krzysztof ; Wcislo, Monika ; Liss, Joanna ; Stachowicz, Anna ; Jakiel, Grzegorz ; Lukaszuk, Mariusz ; Pastuszek, Ewa ; Woclawek-Potocka, Izabela ; Galvao, Antonio ; Bialobrzeska, Dorota</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c391t-f48f973fe65cb0d2f9b5f94ad50741064828578818dbef07a19df15160c957a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Case Report</topic><topic>intracytoplasmic sperm injection</topic><topic>sperm</topic><topic>sperm dna integrity</topic><topic>testicular sperm aspiration</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lukaszuk, Krzysztof</creatorcontrib><creatorcontrib>Wcislo, Monika</creatorcontrib><creatorcontrib>Liss, Joanna</creatorcontrib><creatorcontrib>Stachowicz, Anna</creatorcontrib><creatorcontrib>Jakiel, Grzegorz</creatorcontrib><creatorcontrib>Lukaszuk, Mariusz</creatorcontrib><creatorcontrib>Pastuszek, Ewa</creatorcontrib><creatorcontrib>Woclawek-Potocka, Izabela</creatorcontrib><creatorcontrib>Galvao, Antonio</creatorcontrib><creatorcontrib>Bialobrzeska, Dorota</creatorcontrib><collection>Thieme Connect Journals Open Access</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>American journal of perinatology reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lukaszuk, Krzysztof</au><au>Wcislo, Monika</au><au>Liss, Joanna</au><au>Stachowicz, Anna</au><au>Jakiel, Grzegorz</au><au>Lukaszuk, Mariusz</au><au>Pastuszek, Ewa</au><au>Woclawek-Potocka, Izabela</au><au>Galvao, Antonio</au><au>Bialobrzeska, Dorota</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>First Pregnancy, Somatic and Psychological Status of a 4-Year-Old Child Born following Annexin V TESA Sperm Separation</atitle><jtitle>American journal of perinatology reports</jtitle><addtitle>AJP Rep</addtitle><date>2015-10-01</date><risdate>2015</risdate><volume>5</volume><issue>2</issue><spage>e105</spage><epage>e108</epage><pages>e105-e108</pages><issn>2157-6998</issn><eissn>2157-7005</eissn><abstract>Abstract
Introduction
Sperm DNA integrity is a crucial paternal factor affecting fertilization and pregnancy rates, as well as embryo development.
Case
The present case report describes the successful pregnancy after testicular sperm aspiration (TESA) combined with intracytoplasmic sperm injection (ICSI) (TESA-ICSI) in a couple where the male presented high sperm DNA fragmentation. In order to sort damaged sperm presenting DNA fragmentation, magnetic activated cell sorting (MACS) with annexin V microbeads (MACS Miltenyi Biotec, Teterow, Germany) was used.
Conclusion
The authors present the first description of a successful medical case using TESA-ICSI annexin V sperm sorting. Additionally, a follow-up of the child at the age of 4 years old was done.</abstract><cop>333 Seventh Avenue, New York, NY 10001, USA</cop><pub>Thieme Medical Publishers</pub><pmid>26495164</pmid><doi>10.1055/s-0035-1548726</doi><oa>free_for_read</oa></addata></record> |
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source | PubMed (Medline); Thieme Connect Journals Open Access |
subjects | Case Report intracytoplasmic sperm injection sperm sperm dna integrity testicular sperm aspiration |
title | First Pregnancy, Somatic and Psychological Status of a 4-Year-Old Child Born following Annexin V TESA Sperm Separation |
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