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First Pregnancy, Somatic and Psychological Status of a 4-Year-Old Child Born following Annexin V TESA Sperm Separation

Abstract Introduction  Sperm DNA integrity is a crucial paternal factor affecting fertilization and pregnancy rates, as well as embryo development. Case  The present case report describes the successful pregnancy after testicular sperm aspiration (TESA) combined with intracytoplasmic sperm injection...

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Published in:American journal of perinatology reports 2015-10, Vol.5 (2), p.e105-e108
Main Authors: Lukaszuk, Krzysztof, Wcislo, Monika, Liss, Joanna, Stachowicz, Anna, Jakiel, Grzegorz, Lukaszuk, Mariusz, Pastuszek, Ewa, Woclawek-Potocka, Izabela, Galvao, Antonio, Bialobrzeska, Dorota
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container_end_page e108
container_issue 2
container_start_page e105
container_title American journal of perinatology reports
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creator Lukaszuk, Krzysztof
Wcislo, Monika
Liss, Joanna
Stachowicz, Anna
Jakiel, Grzegorz
Lukaszuk, Mariusz
Pastuszek, Ewa
Woclawek-Potocka, Izabela
Galvao, Antonio
Bialobrzeska, Dorota
description Abstract Introduction  Sperm DNA integrity is a crucial paternal factor affecting fertilization and pregnancy rates, as well as embryo development. Case  The present case report describes the successful pregnancy after testicular sperm aspiration (TESA) combined with intracytoplasmic sperm injection (ICSI) (TESA-ICSI) in a couple where the male presented high sperm DNA fragmentation. In order to sort damaged sperm presenting DNA fragmentation, magnetic activated cell sorting (MACS) with annexin V microbeads (MACS Miltenyi Biotec, Teterow, Germany) was used. Conclusion  The authors present the first description of a successful medical case using TESA-ICSI annexin V sperm sorting. Additionally, a follow-up of the child at the age of 4 years old was done.
doi_str_mv 10.1055/s-0035-1548726
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subjects Case Report
intracytoplasmic sperm injection
sperm
sperm dna integrity
testicular sperm aspiration
title First Pregnancy, Somatic and Psychological Status of a 4-Year-Old Child Born following Annexin V TESA Sperm Separation
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