Spatial Distribution and Enrichment Dynamics of Foodborne Norovirus in Oyster Tissues

The prevalence of norovirus in oysters poses a significant threat to food safety, necessitating a comprehensive understanding of contamination patterns. This study explores the temporal dynamics of norovirus distribution in various oyster tissues over a contamination period ranging from 6 to 96 h. F...

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Bibliographic Details
Published in:Foods 2023-12, Vol.13 (1), p.128
Main Authors: Mao, Mao, Zhang, Zilei, Zhao, Xuchong, Geng, Haoran, Xue, Liang, Liu, Danlei
Format: Article
Language:English
Subjects:
Adsorption
Circulatory system
Contamination
detection strategies
Digestive glands
Fishing
Food consumption
Food contamination
Food contamination & poisoning
Food safety
Foodborne diseases
foodborne infections
Gastroenteritis
Genomes
Glands
Health aspects
Medical research
Medicine, Experimental
Monitoring
norovirus contamination
oyster tissues
Oysters
Public health
Shellfish
Spatial distribution
Stomach
temporal dynamics
Tissues
Viruses
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Summary:The prevalence of norovirus in oysters poses a significant threat to food safety, necessitating a comprehensive understanding of contamination patterns. This study explores the temporal dynamics of norovirus distribution in various oyster tissues over a contamination period ranging from 6 to 96 h. Four tissues-the gill, palp, digestive gland, and stomach-were subjected to systematic monitoring using RT-qPCR for absolute quantification. Results revealed rapid norovirus detection in all tissues six hours post-contamination, with subsequent variations in detection rates. Gill and digestive gland tissues exhibited a peak in detection at 12-24 h, aligning with the oyster's gastrointestinal circulatory system. The digestive gland, distinguished by specific enrichment and adsorption capabilities, demonstrated the highest virus concentration at 48 h. In contrast, the stomach displayed a reemergence of norovirus. Beyond 72 h, detection remained exclusive to the digestive gland, with Ct values comparable to earlier time points. At 96 h, a limited amount of norovirus was detected in the digestive gland, emphasizing the importance for timely monitoring. In addition to providing critical insights into optimal detection strategies, these findings highlight the time-related characteristics of norovirus contamination in oysters. The study identifies the digestive gland as a key target for reliable monitoring, providing valuable data to improve protocols for reducing hazards associated with oyster consumption and foodborne norovirus infections. This research contributes to the understanding of norovirus dynamics in oyster tissues and reinforces current efforts aimed at ensuring food safety and public health.
ISSN:2304-8158
2304-8158
DOI:10.3390/foods13010128