Assessment of a DNA vaccine encoding an anchored-glycosylphosphatidylinositol tegumental antigen complexed to protamine sulphate on immunoprotection against murine schistosomiasis

Protamine sulphate/DNA complexes have been shown to protect DNA from DNase digestion in a lipid system for gene transfer. A DNA-based vaccine complexed to protamine sulphate was used to induce an immune response against Schistosoma mansoni anchored-glycosylphosphatidylinositol tegumental antigen in...

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Bibliographic Details
Published in:Memórias do Instituto Oswaldo Cruz 2007-02, Vol.102 (1), p.21-27
Main Authors: Nascimento, Eduardo JM, Amorim, Rosa V, Cavalcanti, André, Alves, Veruska F, Nakazawa, Mineo, Pereira, Valéria RA, Lucena-Silva, Norma
Format: Article
Language:English
Subjects:
Animals
Antibodies, Helminth - blood
Antibodies, Helminth - immunology
Antigens, Helminth - administration & dosage
Antigens, Helminth - immunology
DNA vaccines
DNA vaccines - pECL - protamine sulphate - schistosomiasis
Female
Glycosylphosphatidylinositols - administration & dosage
Glycosylphosphatidylinositols - immunology
Heparin Antagonists - administration & dosage
Heparin Antagonists - immunology
Immunoglobulin G - immunology
Mice
Mice, Inbred BALB C
PARASITOLOGY
pECL
protamine sulphate
Protamines - administration & dosage
Protamines - immunology
Schistosoma mansoni - immunology
schistosomiasis
Schistosomiasis mansoni - prevention & control
Time Factors
TROPICAL MEDICINE
Vaccines, DNA - administration & dosage
Vaccines, DNA - immunology
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Summary:Protamine sulphate/DNA complexes have been shown to protect DNA from DNase digestion in a lipid system for gene transfer. A DNA-based vaccine complexed to protamine sulphate was used to induce an immune response against Schistosoma mansoni anchored-glycosylphosphatidylinositol tegumental antigen in BALB/c mice. The protection elicited ranged from 33 to 44%. The spectrum of the elicited immune response induced by the vaccine formulation without protamine was characterized by a high level of IgG (IgG1 > IgG2a). Protamine sulphate added to the DNA vaccine formulation retained the green fluorescent protein encoding-plasmid longer in muscle and spleen. The experiments in vivo showed that under protamine sulphate effect, the scope of protection remained unchanged, but a modulation in antibody production (IgG1 = IgG2a) was observed.
ISSN:1678-8060
0074-0276
0074-0276
DOI:10.1590/S0074-02762007000100003