Beneficial Effects of Catalpol Supplementation during In Vitro Maturation of Porcine Cumulus-Oocyte Complexes

Oxidative stress degrades oocytes during in vitro maturation (IVM). Catalpol, a well-known iridoid glycoside, exhibits antioxidant, anti-inflammatory, and antihyperglycemic effects. In this study, catalpol supplementation was tested on porcine oocyte IVM and its mechanisms. Corticalgranule (GC) dist...

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Published in:Antioxidants 2023-06, Vol.12 (6), p.1222
Main Authors: Wang, Yanxin, Xu, Ye, Li, Sijia, Yan, Xi, Yang, Xiaofen, Chen, Mengjia, Wang, Yun, Jia, Ruru, Zhou, Dongping, Shi, Deshun, Lu, Fenghua
Format: Article
Language:English
Subjects:
Analysis
antioxidant
Antioxidants
Biotechnology
catalpol
Cell number
DNA damage
Embryogenesis
Embryonic development
Ethylenediaminetetraacetic acid
Glutathione
Homeostasis
in vitro maturation
Inflammation
Maturation
Membrane potential
Mitochondria
Mitochondrial DNA
Oocytes
Ovaries
Oxidative stress
Reactive oxygen species
Reagents
Supplements
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Summary:Oxidative stress degrades oocytes during in vitro maturation (IVM). Catalpol, a well-known iridoid glycoside, exhibits antioxidant, anti-inflammatory, and antihyperglycemic effects. In this study, catalpol supplementation was tested on porcine oocyte IVM and its mechanisms. Corticalgranule (GC) distribution, mitochondrial function, antioxidant capacity, DNA damage degree, and real-time quantitative polymerase chain reaction were used to confirm the effects of 10 μmol/L catalpol in the maturation medium during IVM. Catalpol treatment significantly increased the first-pole rate and cytoplasmic maturation in mature oocytes. It also increased oocyte glutathione (GSH), mitochondrial membrane potential and blastocyst cell number. However, DNA damage as well as reactive oxygen species (ROS) and malondialdehyde (MDA) levels. Mitochondrial membrane potential and blastocyst cell number were also increased. Thus, the supplementation of 10 μmol/L catalpol in the IVM medium improves porcine oocyte maturation and embryonic development.
ISSN:2076-3921
2076-3921
DOI:10.3390/antiox12061222