Influence of the 2000-m ergometer test on indirect markers of intestinal injury in competitive elite rowers in different training phases

• Published in: BMC sports science, medicine & rehabilitation medicine & rehabilitation, 2023-11, Vol.15 (1), p.1-148, Article 148
• Main Authors: Dziewiecka, Hanna, Kasperska, Anna, Ostapiuk-Karolczuk, Joanna, CichoÅ-Woźniak, Justyna, Basta, Piotr, SkarpaÅska-Stejnborn, Anna
• Format: Article
• Language: English
• Subjects: Athletes; Endurance; Fatty acids; Gut injury; I-FABP; Interleukins; LPS; Permeability; Protein binding; Proteins; Rowers; Rowing; Statistical analysis; Variance analysis; Workloads; Zonulin
• ISSN: 2052-1847; 2052-1847
• DOI: 10.1186/s13102-023-00761-w

Background We examined the effect of the 2000-m ergometer test on gut injury in competitive elite rowers in two different training phases. Given that inflammatory markers during the competitive phase are higher, we hypothesise that markers of intestinal injury are also more elevated during that phase. Methods We performed this study during the preparatory phase (Test I) and competitive phase (Test II) of annual training. We included 10 competitive elite rowers, members of the Polish Rowing Team, in the study after applying the inclusion/exclusion criteria. The participants performed a 2000-m ergometer test during both phases (Tests I and II). We collected blood samples before the test, immediately after the test and after 1 h of recovery. We measured the levels of interleukin 6 (IL-6), intestinal fatty acid binding protein (I-FABP), lipopolysaccharide (LPS), lipopolysaccharide-binding protein (LBP), and zonulin. Results There were no significant changes over time in Test I and Test II in the gut integrity markers. There were significantly lower I-FABP and IL-6 levels after the test for Test II compared with Test I. The pre-test LPS level was significantly lower for Test II compared with Test I. The pre-test LBP and zonulin levels were numerically lower in Test II, but the differences were not significant. Conclusions The 2000-m ergometer test showed no influence on gut integrity markers. However, there were differences in the response to exercise between Tests I and II. The lower level of gut injury markers after extreme exercise tests carried out during the preparation period may be the result of adaptive mechanisms and could indicate that rationally conducted training significantly decreases intestinal injury. Keywords: Athletes, Rowers, Gut injury, I-FABP, Zonulin, LPS, LBP, Il-6, Preparation phase