Identification and Analysis of Stress-Associated Proteins (SAPs) Protein Family and Drought Tolerance of ZmSAP8 in Transgenic Arabidopsis

Stress-associated proteins (SAPs), a class of A20/AN1 zinc finger proteins, play vital roles in plant stress response. However, investigation of SAPs in maize has been very limited. Herein, to better trace the evolutionary history of SAPs in maize and plants, 415 SAPs were identified in 33 plant spe...

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Published in:International journal of molecular sciences 2022-11, Vol.23 (22), p.14109
Main Authors: Su, Anqi, Qin, Qianqian, Liu, Chao, Zhang, Jiajun, Yu, Bingxin, Cheng, Yifeng, Wang, Sijia, Tang, Jiawen, Si, Weina
Format: Article
Language:English
Subjects:
A20/AN1 domain
Abiotic stress
Angiosperms
Arabidopsis - metabolism
Chromosomes
Corn
Drought
Drought resistance
Droughts
Evolution
Flowers & plants
Food security
Gene duplication
Gene Expression Regulation, Plant
gene family
Genes
Genomes
Germination
Heat-Shock Proteins - metabolism
High temperature
maize
Pattern analysis
Phylogeny
Plant resistance
Plant stress
Plants (botany)
Proteins
Reproduction (copying)
Rice
SAP gene
Seed germination
Stress analysis
Stress, Physiological - genetics
stress-associated protein
Zea mays - genetics
Zea mays - metabolism
Zinc
Zinc Fingers - genetics
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Summary:Stress-associated proteins (SAPs), a class of A20/AN1 zinc finger proteins, play vital roles in plant stress response. However, investigation of SAPs in maize has been very limited. Herein, to better trace the evolutionary history of SAPs in maize and plants, 415 SAPs were identified in 33 plant species and four species of other kingdoms. Moreover, gene duplication mode exploration showed whole genome duplication contributed largely to SAP gene expansion in angiosperms. Phylogeny reconstruction was performed with all identified SAPs by the maximum likelihood (ML) method and the SAPs were divided into five clades. SAPs within the same clades showed conserved domain composition. Focusing on maize, nine were identified. Further promoter cis-elements and stress-induced expression pattern analysis of indicated that was a promising candidate in response to drought stress, which was the only AN1-AN1-C2H2-C2H2 type SAP in maize and belonged to clade I. Additionally, ZmSAP8 was located in the nucleus and had no transactivation activity in yeast. Overexpressing enhanced the tolerance to drought stress in , with higher seed germination and longer root length. Our results should benefit the further functional characterization of .
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms232214109