Isochoric Supercooling Organ Preservation System

This technical paper introduces a novel organ preservation system based on isochoric (constant volume) supercooling. The system is designed to enhance the stability of the metastable supercooling state, offering potential long-term preservation of large biological organs at subfreezing temperatures...

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Bibliographic Details
Published in:Bioengineering (Basel) 2023-08, Vol.10 (8), p.934
Main Authors: Năstase, Gabriel, Botea, Florin, Beșchea, George-Andrei, Câmpean, Ștefan-Ioan, Barcu, Alexandru, Neacșu, Ion, Herlea, Vlad, Popescu, Irinel, Chang, Tammy T, Rubinsky, Boris, Șerban, Alexandru
Format: Article
Language:English
Subjects:
Additives
Bioengineering
Blood vessels
Cold storage
Cryopreservation
Cryoprotectants
Cryoprotectors
Equilibrium
Freezing
Ice nucleation
isochoric system
Kidneys
large organ preservation
Liver
Metabolism
Nucleation
Nutrients
Organs
Preservation
subfreezing temperatures
Success
Supercooling
Technical papers
Temperature
Transplants & implants
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Summary:This technical paper introduces a novel organ preservation system based on isochoric (constant volume) supercooling. The system is designed to enhance the stability of the metastable supercooling state, offering potential long-term preservation of large biological organs at subfreezing temperatures without the need for cryoprotectant additives. Detailed technical designs and usage protocols are provided for researchers interested in exploring this field. The paper also presents a control system based on the thermodynamics of isochoric freezing, utilizing pressure monitoring for process control. Sham experiments were performed using whole pig liver sourced from a local food supplier to evaluate the system’s ability to sustain supercooling without ice nucleation for extended periods. The results demonstrated sustained supercooling without ice nucleation in pig liver tissue for 24 and 48 h. These findings suggest the potential of this technology for large-volume, cryoprotectant-free organ preservation with real-time control over the preservation process. The simplicity of the isochoric supercooling device and the design details provided in the paper are expected to serve as encouragement for other researchers in the field to pursue further research on isochoric supercooling. However, final evidence that these preserved organs can be successfully transplanted is still lacking.
ISSN:2306-5354
2306-5354
DOI:10.3390/bioengineering10080934