Characterization of Differentially Expressed miRNAs by CXCL12/SDF-1 in Human Bone Marrow Stromal Cells

Stromal cell-derived factor 1 (SDF-1) is known to influence bone marrow stromal cell (BMSC) migration, osteogenic differentiation, and fracture healing. We hypothesize that SDF-1 mediates some of its effects on BMSCs through epigenetic regulation, specifically via microRNAs (miRNAs). MiRNAs are smal...

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Published in:Biomolecular concepts 2021-10, Vol.12 (1), p.132-143
Main Authors: Potter, Matthew L., Smith, Kathryn, Vyavahare, Sagar, Kumar, Sandeep, Periyasamy-Thandavan, Sudharsan, Hamrick, Mark, Isales, Carlos M., Hill, William D., Fulzele, Sadanand
Format: Article
Language:English
Subjects:
Bioinformatics
Biosynthesis
Bone healing
Bone marrow
bone marrow stromal cells
Cell differentiation
Cell migration
CXCL12 protein
Epigenetics
Fractures
Gene expression
MicroRNAs
miRNA
Mucin
SDF-1
SDF-1 protein
Stem cells
Stromal cells
Thyroid
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cited_by cdi_FETCH-LOGICAL-c3932-8352d82cc9210aaac6fd0c84892118b5361968430f58325c8b76a551ec90a5843
cites cdi_FETCH-LOGICAL-c3932-8352d82cc9210aaac6fd0c84892118b5361968430f58325c8b76a551ec90a5843
container_end_page 143
container_issue 1
container_start_page 132
container_title Biomolecular concepts
container_volume 12
creator Potter, Matthew L.
Smith, Kathryn
Vyavahare, Sagar
Kumar, Sandeep
Periyasamy-Thandavan, Sudharsan
Hamrick, Mark
Isales, Carlos M.
Hill, William D.
Fulzele, Sadanand
description Stromal cell-derived factor 1 (SDF-1) is known to influence bone marrow stromal cell (BMSC) migration, osteogenic differentiation, and fracture healing. We hypothesize that SDF-1 mediates some of its effects on BMSCs through epigenetic regulation, specifically via microRNAs (miRNAs). MiRNAs are small non-coding RNAs that target specific mRNA and prevent their translation. We performed global miRNA analysis and determined several miRNAs were differentially expressed in response to SDF-1 treatment. Gene Expression Omnibus (GEO) dataset analysis showed that these miRNAs play an important role in osteogenic differentiation and fracture healing. KEGG and GO analysis indicated that SDF-1 dependent miRNAs changes affect multiple cellular pathways, including fatty acid biosynthesis, thyroid hormone signaling, and mucin-type O-glycan biosynthesis pathways. Furthermore, bioinformatics analysis showed several miRNAs target genes related to stem cell migration and differentiation. This study's findings indicated that SDF-1 induces some of its effects on BMSCs function through miRNA regulation.
doi_str_mv 10.1515/bmc-2021-0015
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source Sciendo (De Gruyter) Open Access Journals
subjects Bioinformatics
Biosynthesis
Bone healing
Bone marrow
bone marrow stromal cells
Cell differentiation
Cell migration
CXCL12 protein
Epigenetics
Fractures
Gene expression
MicroRNAs
miRNA
Mucin
SDF-1
SDF-1 protein
Stem cells
Stromal cells
Thyroid
title Characterization of Differentially Expressed miRNAs by CXCL12/SDF-1 in Human Bone Marrow Stromal Cells
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