Characterization of bacterial communities associated with blood-fed and starved tropical bed bugs, Cimex hemipterus (F.) (Hemiptera): a high throughput metabarcoding analysis

With the development of new metagenomic techniques, the microbial community structure of common bed bugs, Cimex lectularius , is well-studied, while information regarding the constituents of the bacterial communities associated with tropical bed bugs, Cimex hemipterus , is lacking. In this study, th...

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Bibliographic Details
Published in:Scientific reports 2021-04, Vol.11 (1), p.8465-8465, Article 8465
Main Authors: Lim, Li, Ab Majid, Abdul Hafiz
Format: Article
Language:English
Subjects:
631/326/2565
631/601
631/601/1466
Animals
Bacteria
Bacteria - classification
Bacteria - genetics
Bacteria - isolation & purification
Bed load
Bedbugs - microbiology
Blood
Blood - microbiology
Cimex hemipterus
Cimicidae
Community structure
DNA - analysis
DNA - genetics
DNA Barcoding, Taxonomic
Feeding Behavior
Humanities and Social Sciences
Insects
Metagenomics
multidisciplinary
rRNA 16S
Science
Science (multidisciplinary)
Starvation
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Summary:With the development of new metagenomic techniques, the microbial community structure of common bed bugs, Cimex lectularius , is well-studied, while information regarding the constituents of the bacterial communities associated with tropical bed bugs, Cimex hemipterus , is lacking. In this study, the bacteria communities in the blood-fed and starved tropical bed bugs were analysed and characterized by amplifying the v3-v4 hypervariable region of the 16S rRNA gene region, followed by MiSeq Illumina sequencing. Across all samples, Proteobacteria made up more than 99% of the microbial community. An alpha-proteobacterium Wolbachia and gamma-proteobacterium, including Dickeya chrysanthemi and Pseudomonas , were the dominant OTUs at the genus level. Although the dominant OTUs of bacterial communities of blood-fed and starved bed bugs were the same, bacterial genera present in lower numbers were varied. The bacteria load in starved bed bugs was also higher than blood-fed bed bugs.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-021-87946-w