Anti-Melanogenesis Effect of Rosa rugosa on α-MSH-Induced B16F10 Cells via PKA/CREB Pathway Activation

• Published in: Applied sciences 2023-01, Vol.13 (1), p.184
• Main Authors: Zhou, Xianrong, Oh, Jung Hwan, Karadeniz, Fatih, Yang, Jiho, Lee, Hyunjung, Seo, Youngwan, Kong, Chang-Suk
• Format: Article
• Language: English
• Subjects: Adenosine; Antibiotics; B16F10 melanoma cells; Cyclic AMP response element-binding protein; Cytotoxicity; Gallic acid; Gene expression; Genes; Hexanes; Hyperpigmentation; Kinases; Melanin; melanogenesis; Melanoma; Microphthalmia-associated transcription factor; Phosphorylation; Pigmentation; PKA/CREB signaling pathway; Polyphenols; Protein kinase A; Proteins; Reagents; Rosa rugosa; Signal transduction; Skin cancer; Solvents; Tumors; Tyrosinase; α-MSH
• ISSN: 2076-3417; 2076-3417
• DOI: 10.3390/app13010184

Melanin protects the skin against UV damage, whereas excessive melanin accumulation causes abnormal pigmentation and even melanoma. It has been reported that Rosa rugosa exhibits antioxidant, anti-bacterial, anti-tumor, and anti-inflammation activities. The current study evaluated the melanogenesis-suppressing effect of R. rugosa extract and its solvent fractions (H2O, n-BuOH, 85% aq. MeOH, and n-hexane). The effect of R. rugosa on the extra/intra-cellular melanin and intracellular active tyrosinase levels, melanogenesis-related gene and protein expression, and PKA/CREB signaling pathway activation was investigated in α-MSH-induced B16F10 cells. The results showed that R. rugosa effectively suppressed melanin secretion and tyrosinase activity at non-cytotoxic concentrations. R. rugosa extract down-regulated the melanogenesis-related expression of genes and proteins of tyrosinase, microphthalmia-associated transcription factor (MITF), tyrosinase-related protein (TRP)-1, and TRP-2. Furthermore, R. rugosa effectively inhibited the phosphorylation of the PKA/CREB proteins. Finally, the total polyphenol content of R. rugosa crude extract and its H2O, n-BuOH, 85% aq. MeOH, and n-hexane solvent fractions were 1383.9 ± 44.5, 2004.7 ± 43.4, 7270.3 ± 54.5, 2064.1 ± 34.8, and 1091.1 ± 26.2 mg gallic acid equivalent/100 g extract, respectively. The anti-melanogenesis effect of R. rugosa was suggested to be exhibited by downregulating the PKA/CREB signaling pathway potentially due to a high content of polyphenols. Overall, R. rugosa crude extract and its solvent fractions could be considered sources of bioactive ingredients that can be used against hyperpigmentation.