Preventive Potential of Resveratrol in Carcinogen-Induced Rat Thyroid Tumorigenesis

• Published in: Nutrients 2018-02, Vol.10 (3), p.279
• Main Authors: Zheng, Xu, Jia, Bin, Song, Xue, Kong, Qing-You, Wu, Mo-Li, Qiu, Ze-Wen, Li, Hong, Liu, Jia
• Format: Article
• Language: English
• Subjects: Adenoma - blood; Adenoma - chemically induced; Adenoma - pathology; Adenoma - prevention & control; Animals; Anticarcinogenic Agents - pharmacology; Carcinoembryonic Antigen - blood; Carcinogenesis; Carcinogens; Cell Line, Tumor; Cell Transformation, Neoplastic - chemically induced; Cell Transformation, Neoplastic - drug effects; Cell Transformation, Neoplastic - metabolism; Cell Transformation, Neoplastic - pathology; chemoprevention; Cyclooxygenase 2 - metabolism; Cyclooxygenase-2; Diethylnitrosamine; Embryos; Humans; Hyperplasia; Immunoglobulins; Injection; Interleukin 6; Interleukin-6 - metabolism; Lesions; Male; Malignancy; Methylnitrosourea; NF-KappaB Inhibitor alpha - metabolism; NF-κB protein; Nitrosamines; Nuclear transport; Rats; Rats, Sprague-Dawley; Resveratrol; Rodents; Stilbenes - pharmacology; Thyroglobulin; Thyroglobulin - blood; Thyroid cancer; Thyroid gland; Thyroid Gland - drug effects; Thyroid Gland - metabolism; Thyroid Gland - pathology; Thyroid Neoplasms - blood; Thyroid Neoplasms - chemically induced; Thyroid Neoplasms - pathology; Thyroid Neoplasms - prevention & control; thyroid tumorigenesis; Time Factors; Tissues; Transcription Factor RelA - metabolism; Translocation; Tumorigenesis
• ISSN: 2072-6643; 2072-6643
• DOI: 10.3390/nu10030279

Thyroid cancer (TC) is the most common endocrine malignancy without reliable preventive agent. Resveratrol possesses in vitro anti-TC activities; while its effect(s) on thyroid tumorigenesis remains unknown. This study aims to address this issue using DEN/MNU/DHPN-induced rat carcinogenesis model. 50 male Sprague-Dawley rats were separated into four groups as Group-1 (5 rats); normally fed; Group-2 (15 rats); DEN/MNU/DHPN treatment only; Group-3 (15 rats) and -4 (15 rats); DEN/MNU/DHPN treatment; followed by resveratrol intragastric (IG) injection and intraperitoneal (IP) injection; respectively; in two-day intervals for 30 weeks. The results revealed that the average resveratrol concentration in thyroid tissues was 1.278 ± 0.419 nmol/g in IG group and 1.752 ± 0.398 nmol/g in IP group. The final body weights of Group-3 and Group-4 were lighter than that ( > 0.05) of Group-1; but heavier than Group-2 ( < 0.05). TC-related lesions (hyperplasia and adenomas) were found in 53.3% of Group-2; 33.3% Group-3 and 26.7% Group-4. Lower serum carcino-embryonic antigen (CEA) and thyroglobulin (Tg) levels; down-regulated expression of IL-6 and cyclooxygenase-2 (COX-2); reduction of NF-κB/p65 nuclear translocation; and elevated IkB expression were found in the thyroid tissues of Group-3 and Group-4 in comparison with that of Group-2. These results demonstrate that IG and IP administered resveratrol efficiently reduces the frequency and severity of DEN/MNU/DHPN-caused TC-related lesions and would be of values in thyroid tumor prevention.