Probiotic Properties and Safety Evaluation of Lactobacillus plantarum HY7718 with Superior Storage Stability Isolated from Fermented Squid

• Published in: Microorganisms (Basel) 2023-09, Vol.11 (9), p.2254
• Main Authors: Kim, Hyeonji, Yoo, Myeong-Seok, Jeon, Hyejin, Shim, Jae-Jung, Park, Woo-Jung, Kim, Joo-Yun, Lee, Jung-Lyoul
• Format: Article
• Language: English
• Subjects: Aluminum; Antibiotics; Bacteria; Dietary supplements; Epithelial cells; Epithelium; Fermentation; Fermented food; fermented foods; Food; Food industry; Gene sequencing; Genomes; Humidity; Industrial safety; Inflammation; Interleukin 6; Intestine; Kinases; Lactic acid; Lactic acid bacteria; Lactobacilli; Lactobacillus plantarum; Microorganisms; Myosin; Myosin-light-chain kinase; Nuclear safety; Nucleotide sequence; Probiotics; Proteins; Reactive oxygen species; Shelf life; Stability analysis; Storage conditions; Storage stability; Temperature; tight junction; Traditional foods; Virulence; Whole genome sequencing; Zonula occludens-1 protein
• ISSN: 2076-2607; 2076-2607
• DOI: 10.3390/microorganisms11092254

The aim of this study was to identify new potential probiotics with improved storage stability and to evaluate their efficacy and safety. Sixty lactic acid bacteria strains were isolated from Korean traditional fermented foods, and their survival was tested under extreme conditions. Lactobacillus plantarum HY7718 (HY7718) showed the greatest stability during storage. HY7718 also showed a stable growth curve under industrial conditions. Whole genome sequencing revealed that the HY7718 genome comprises 3.26 Mbp, with 44.5% G + C content, and 3056 annotated Protein-coding DNA sequences (CDSs). HY7718 adhered to intestinal epithelial cells and was tolerant to gastric fluids. Additionally, HY7718 exhibited no hemolytic activity and was not resistant to antibiotics, confirming that it has probiotic properties and is safe for consumption. Additionally, we evaluated its effects on intestinal health using TNF-induced Caco-2 cells. HY7718 restored the expression of tight junction proteins such as zonular occludens (ZO-1, ZO-2), occludin (OCLN), and claudins (CLDN1, CLDN4), and regulated the expression of myosin light-chain kinase (MLCK), Elk-1, and nuclear factor kappa B subunit 1 (NFKB1). Moreover, HY7718 reduced the secretion of proinflammatory cytokines such as interleukin-6 (IL-6) and IL-8, as well as reducing the levels of peroxide-induced reactive oxygen species. In conclusion, HY7718 has probiotic properties, is safe, is stable under extreme storage conditions, and exerts positive effects on intestinal cells. These results suggest that L. plantarum HY7718 is a potential probiotic for use as a functional supplement in the food industry.