Electrochemical Biosensors Based on Membrane-Bound Enzymes in Biomimetic Configurations

In nature, many enzymes are attached or inserted into the cell membrane, having hydrophobic subunits or lipid chains for this purpose. Their reconstitution on electrodes maintaining their natural structural characteristics allows for optimizing their electrocatalytic properties and stability. Differ...

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Bibliographic Details
Published in:Sensors (Basel, Switzerland) Switzerland), 2020-06, Vol.20 (12), p.3393
Main Authors: Alvarez-Malmagro, Julia, García-Molina, Gabriel, López De Lacey, Antonio
Format: Article
Language:English
Subjects:
Biomimetic Materials
biomimetic membranes
Biomimetics
Biosensing Techniques - instrumentation
biosensor
Biosensors
Cell Membrane
Electric fields
Electrochemical Techniques - instrumentation
Electrodes
Enzymes
Enzymes, Immobilized - chemistry
Gold
Hydrophobicity
Lipid Bilayers
Lipids
membrane-bound enzymes
Membranes
Methods
Proteins
Review
Self-assembled monolayers
Self-assembly
Spectrum analysis
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Summary:In nature, many enzymes are attached or inserted into the cell membrane, having hydrophobic subunits or lipid chains for this purpose. Their reconstitution on electrodes maintaining their natural structural characteristics allows for optimizing their electrocatalytic properties and stability. Different biomimetic strategies have been developed for modifying electrodes surfaces to accommodate membrane-bound enzymes, including the formation of self-assembled monolayers of hydrophobic compounds, lipid bilayers, or liposomes deposition. An overview of the different strategies used for the formation of biomimetic membranes, the reconstitution of membrane enzymes on electrodes, and their applications as biosensors is presented.
ISSN:1424-8220
1424-8220
DOI:10.3390/s20123393