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Pathogenicity and phenotypic sulfadiazine resistance of Toxoplasma gondii isolates obtained from livestock in northeastern Brazil
Toxoplasma gondii is the causative protozoan agent of toxoplasmosis, which is a common infection that is widely distributed worldwide. Studies revealed stronger clonal strains in North America and Europe and genetic diversity in South American strains. Our study aimed to differentiate the pathogenic...
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Published in: | Memórias do Instituto Oswaldo Cruz 2016-06, Vol.111 (6), p.391-398 |
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Main Authors: | , , , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | Toxoplasma gondii is the causative protozoan agent of toxoplasmosis,
which is a common infection that is widely distributed worldwide.
Studies revealed stronger clonal strains in North America and Europe
and genetic diversity in South American strains. Our study aimed to
differentiate the pathogenicity and sulfadiazine resistance of three T.
gondii isolates obtained from livestock intended for human consumption.
The cytopathic effects of the T. gondii isolates were evaluated. The
pathogenicity was determined by polymerase chain reaction-restriction
fragment length polymorphism (PCR-RFLP) using a CS3 marker and in a
rodent model in vivo. Phenotypic sulfadiazine resistance was measured
using a kinetic curve of drug activity in Swiss mice. IgM and IgG were
measured by ELISA, and the dihydropteroate synthase (DHPS) gene
sequence was analysed. The cytopathic effects and the PCR-RFLP profiles
from chickens indicated a different infection source. The Ck3 isolate
displayed more cytopathic effects in vitro than the Ck2 and ME49
strains. Additionally, the Ck2 isolate induced a differential humoral
immune response compared to ME49. The Ck3 and Pg1 isolates, but not the
Ck2 isolate, showed sulfadiazine resistance in the sensitivity assay.
We did not find any DHPS gene polymorphisms in the mouse samples. These
atypical pathogenicity and sulfadiazine resistance profiles were not
previously reported and served as a warning to local health
authorities. |
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ISSN: | 1678-8060 0074-0276 1678-8060 |
DOI: | 10.1590/0074-02760150459 |