Micrometam C Protects against Oxidative Stress in Inflammation Models in Zebrafish and RAW264.7 Macrophages

Micrometam C is a core of novel marine compound isolated from the mangrove associates Micromelum falcatum. In this study, we investigated the protective effects of micrometam C in inflammation models in the transgenic zebrafish line Tg (corola: eGFP) and RAW264.7 macrophages. We found that micrometa...

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Bibliographic Details
Published in:Marine drugs 2015-08, Vol.13 (9), p.5593-5605
Main Authors: Tang, Hao, Ge, Hui, Chen, Zhi-Bin, Luo, Xiong-Ming, Su, Feng-Juan, Liang, Yan-Bing, Li, Zhen-Yu, Wu, Jing-Guo, Yang, Qing, Zeng, Li-Jin, Ma, Zhong-Fu
Format: Article
Language:English
Subjects:
Animals
Anti-Inflammatory Agents - chemistry
Anti-Inflammatory Agents - pharmacology
Antioxidants
Cell Line
Coumaric Acids - chemistry
Coumaric Acids - pharmacology
Danio rerio
Gene Expression Regulation - drug effects
Inflammation - drug therapy
inflammatory
Lipopolysaccharides - toxicity
Macrophages
Mice
micrometam C
Molecular Structure
NADPH oxidase
NADPH Oxidases - metabolism
NF-kappa B - genetics
NF-kappa B - metabolism
NF-κB
Oxidative Stress
Respiratory Burst
Tail
Zebrafish
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Summary:Micrometam C is a core of novel marine compound isolated from the mangrove associates Micromelum falcatum. In this study, we investigated the protective effects of micrometam C in inflammation models in the transgenic zebrafish line Tg (corola: eGFP) and RAW264.7 macrophages. We found that micrometam C significantly suppressed the migration of immune cells in tail-cutting-induced inflammation in transgenic zebrafish and reduced lipopolysaccharide (LPS)-induced reactive oxygen species (ROS) in both zebrafish and macrophages. In addition, micrometam C also restored LPS-induced reduction of endogenous antioxidants, such as catalase (CAT), glutathione (GSH) and superoxide dismutase (SOD). The protective effects of micrometam C were in parallel to its inhibition of NADPH oxidase and nuclear factor-kappa-binding (NF-κB) activity. Thus, the present results demonstrate that micrometam C protects against LPS-induced inflammation possibly through its antioxidant property.
ISSN:1660-3397
1660-3397
DOI:10.3390/md13095593