Mitochondrial superoxide dismutase overexpression and low oxygen conditioning hormesis improve the performance of irradiated sterile males

• Published in: Scientific reports 2021-10, Vol.11 (1), p.20182-15, Article 20182
• Main Authors: Dias, Vanessa S., Cáceres, Carlos, Parker, Andrew G., Pereira, Rui, Demirbas-Uzel, Güler, Abd-Alla, Adly M. M., Teets, Nicholas M., Schetelig, Marc F., Handler, Alfred M., Hahn, Daniel A.
• Format: Article
• Language: English
• Subjects: 631/158; 631/181; 631/208; 631/601; 631/61; Animal reproduction; Animals; Animals, Genetically Modified; Antioxidants; Atmospheric conditions; Competitiveness; Domestication; Hormesis; Humanities and Social Sciences; Hypoxia; Infertility, Male - etiology; Insect Control - methods; Insect Proteins - genetics; Insect Proteins - metabolism; Insects; Ionizing radiation; Irradiation; Lek behavior; Male; Male sterility; Males; Mass rearing; Mating; Mitochondria; multidisciplinary; Mutation; Oxidative Stress; Oxygen; Oxygen - metabolism; Pest control; Science; Science (multidisciplinary); Sexual behavior; Sexual Behavior, Animal - physiology; Sexual Behavior, Animal - radiation effects; Sterilized organisms; Superoxide dismutase; Superoxide Dismutase - genetics; Superoxide Dismutase - metabolism; Tephritidae - enzymology; Tephritidae - physiology; Tephritidae - radiation effects
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-021-99594-1

The Sterile Insect Technique (SIT) is a successful autocidal control method that uses ionizing radiation to sterilize insects. However, irradiation in normal atmospheric conditions can be damaging for males, because irradiation generates substantial biological oxidative stress that, combined with domestication and mass-rearing conditions, may reduce sterile male sexual competitiveness and quality. In this study, biological oxidative stress and antioxidant capacity were experimentally manipulated in Anastrepha suspensa using a combination of low-oxygen conditions and transgenic overexpression of mitochondrial superoxide dismutase (SOD2) to evaluate their role in the sexual behavior and quality of irradiated males. Our results showed that SOD2 overexpression enhances irradiated insect quality and improves male competitiveness in leks. However, the improvements in mating performance were modest, as normoxia-irradiated SOD2 males exhibited only a 22% improvement in mating success compared to normoxia-irradiated wild type males. Additionally, SOD2 overexpression did not synergistically improve the mating success of males irradiated in either hypoxia or severe hypoxia. Short-term hypoxic and severe-hypoxic conditioning hormesis, per se, increased antioxidant capacity and enhanced sexual competitiveness of irradiated males relative to non-irradiated males in leks. Our study provides valuable new information that antioxidant enzymes, particularly SOD2, have potential to improve the quality and lekking performance of sterile males used in SIT programs.