An Effective Method of Isolating Honey Proteins

Honey is a natural sweetener composed mostly of sugars, but it contains also pollen grains, proteins, free amino acids, and minerals. The amounts and proportions of these components depend on the honey type and bee species. Despite the low content of honey protein, they are becoming a popular study...

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Bibliographic Details
Published in:Molecules (Basel, Switzerland) Switzerland), 2019-06, Vol.24 (13), p.2399
Main Authors: Bocian, Aleksandra, Buczkowicz, Justyna, Jaromin, Marcin, Hus, Konrad Kamil, Legáth, Jaroslav
Format: Article
Language:English
Subjects:
Brassica napus - metabolism
Dialysis
Distilled water
Electrophoresis
Electrophoresis, Gel, Two-Dimensional
Fagopyrum - metabolism
Freeze drying
Gel electrophoresis
Hemodialysis
Honey
Honey - analysis
Mass spectrometry
Mass spectroscopy
Methods
Minerals
Molecular weight
phenol extraction
Phenols
Phenols - chemistry
Plant Proteins - isolation & purification
Poland
Pollen
Polyacrylamide
Proteins
Quality control
Robinia - metabolism
Sugar
Variance analysis
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Summary:Honey is a natural sweetener composed mostly of sugars, but it contains also pollen grains, proteins, free amino acids, and minerals. The amounts and proportions of these components depend on the honey type and bee species. Despite the low content of honey protein, they are becoming a popular study object, and have recently been used as markers of the authenticity and quality of honey. Currently, the most popular methods of protein isolation from honey are dialysis against distilled water, lyophilization of dialysate, or various precipitation protocols. In this work, we propose a new method based on saturated phenol. We tested it on three popular polish honey types and we proved its compatibility with both 1D and 2D polyacrylamide gel electrophoresis (PAGE) and MS (mass spectrometry) techniques. The elaborated technique is also potentially less expensive and less time-consuming than other previously described methods, while being equally effective.
ISSN:1420-3049
1420-3049
DOI:10.3390/molecules24132399