Characterization and comparative analysis of the Escherichia marmotae M-12 isolate from bank vole (Myodes glareolus)

The Escherichia marmotae is a bacterium of the Enterobacterales order, which was first isolated from the Himalayan marmot ( Marmota himalayana ). Recently E. marmotae has been shown to cause severe infections in humans. Wild animals were suggested to be a natural reservoir of this bacterium. The pre...

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Published in:Scientific reports 2023-08, Vol.13 (1), p.13949-13949, Article 13949
Main Authors: Zhurilov, Pavel A., Andriyanov, Pavel A., Tutrina, Anastasia I., Razheva, Irina V., Liskova, Elena A., Gladkova, Nadezda A., Kashina, Daria D., Yashin, Ivan V., Blokhin, Andrey A.
Format: Article
Language:English
Subjects:
631/326/1320
631/326/41/2531
Animals
Antibiotics
Autopsies
Comparative analysis
E coli
Enderleinellus marmotae
Erythrocytes
Escherichia
Flagella
Genomes
Genotypes
Humanities and Social Sciences
Infections
Marmota himalayana
Motility
multidisciplinary
Myodes glareolus
Phenotypes
Phylogenetics
Phylogeny
Pili
Science
Science (multidisciplinary)
Sepsis
Siderophores
Virology
Virulence
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Summary:The Escherichia marmotae is a bacterium of the Enterobacterales order, which was first isolated from the Himalayan marmot ( Marmota himalayana ). Recently E. marmotae has been shown to cause severe infections in humans. Wild animals were suggested to be a natural reservoir of this bacterium. The present study describes the first case of E. marmotae isolation from an apparently healthy wild bank vole ( Myodes glareolus ). Phenotype, as well as genotype-based techniques, were applied to characterize E. marmotae M-12 isolate. E. marmotae M-12 had the capsule-positive phenotype, high adhesion to human erythrocytes and HEp-2 cells as well as a low invasion into HEp-2 cells. E. marmotae M-12 was avirulent in mice. The phylogenomic analyses of E. marmotae showed dispersed phylogenetic structure among isolates of different origins. Virulome analysis of M-12 isolate revealed the presence of the following factors: siderophores, heme uptake systems, capsule synthesis, curli and type I fimbriae, flagella proteins, OmpA porin, etc. Comparative virulome analysis among available E. marmotae genomes revealed the presence of capsule K1 genes mostly in pathogenic isolates and OmpA porin presence among all strains. We assume that the K1 capsule and OmpA porin play a key role in the virulence of E. marmotae . Pathogenesis of the latter might be similar to extraintestinal pathogenic E. coli .
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-023-41223-0