Tempol improves lipid profile and prevents left ventricular hypertrophy in LDL receptor gene knockout (LDLr-/-) mice on a high-fat diet

Dyslipidemia is associated with increased risk of cardiovascular disease and atherosclerosis, and hence with high morbidity and mortality. This study investigated the effects of the nitroxide 4-hydroxy-2,2,6,6-tetramethylpiperidine 1-oxyl (Tempol) on lipid profile and cardiac morphology in low-densi...

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Published in:Revista portuguesa de cardiologia 2017-09, Vol.36 (9), p.629-638
Main Authors: Viana Gonçalves, Igor Cândido, Cerdeira, Cláudio Daniel, Poletti Camara, Eduardo, Dias Garcia, José Antônio, Ribeiro Pereira Lima Brigagão, Maísa, Bessa Veloso Silva, Roberta, Bitencourt dos Santos, Gérsika
Format: Article
Language:English
Subjects:
Animals
Cyclic N-Oxides - pharmacology
Cyclic N-Oxides - therapeutic use
Diet, High-Fat
Dislipidemia
Dyslipidemia
EROs/ERNs
Hipertrofia ventricular esquerda
Hypertrophy, Left Ventricular - prevention & control
Left ventricular hypertrophy
Lipid Metabolism - drug effects
Lipids - blood
Male
Mice
Mice, Inbred C57BL
Mice, Knockout
Nitroxides
Nitróxidos
Reactive nitrogen species
Reactive oxygen species
Receptors, LDL - genetics
Spin Labels
Tempol
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Summary:Dyslipidemia is associated with increased risk of cardiovascular disease and atherosclerosis, and hence with high morbidity and mortality. This study investigated the effects of the nitroxide 4-hydroxy-2,2,6,6-tetramethylpiperidine 1-oxyl (Tempol) on lipid profile and cardiac morphology in low-density lipoprotein (LDL) receptor gene knockout (LDLr-/-) mice. Male LDLr-/- mice (three months old, approximately 22 g weight) were divided into the following groups: controls, including (1) standard chow (SC, n=8) and (2) high-fat diet (HFD, n=8); and treatment, including (3) standard chow + Tempol (SC+T, n=8) (30 mg/kg administered by gavage, once daily) and (4) high-fat diet + Tempol (HFD+T, n=8) (30 mg/kg). After 30 days of the diet/treatment, whole blood was collected for analysis of biochemical parameters (total cholesterol, triglycerides [TG], high-density lipoprotein [HDL], LDL, and very low-density lipoprotein [VLDL]). The heart was removed through thoracotomy and histological analysis of the left ventricle was performed. A significant increase in TG, LDL, and VLDL and marked left ventricular hypertrophy (LVH) were demonstrated in the HFD group relative to the SC group (p
ISSN:0870-2551
2174-2030
DOI:10.1016/j.repc.2017.02.014